Fluorescence lifetime spectroscopy of glioblastoma multiforme

Photochemistry and Photobiology
Laura MarcuReid C Thompson

Abstract

Fluorescence spectroscopy of the endogenous emission of brain tumors has been researched as a potentially important method for the intraoperative localization of brain tumor margins. We investigated the use of time-resolved, laser-induced fluorescence spectroscopy for demarcation of primary brain tumors by studying the time-resolved spectra of gliomas. The fluorescence of human brain samples (glioblastoma multiforme, cortex and white matter: six patients, 23 sites) was induced ex vivo with a pulsed nitrogen laser (337 nm, 3 ns). The time-resolved spectra were detected in a 360-550 nm wavelength range using a fast digitizer and gated detection. Parameters derived from both the spectral- (intensities from narrow spectral bands) and the time domain (average lifetime) measured at 390 and 460 nm were used for tissue characterization. We determined that high-grade gliomas are characterized by fluorescence lifetimes that varied with the emission wavelength (>3 ns at 390 nm, <1 ns at 460 nm) and their emission is overall longer than that of normal brain tissue. Our study demonstrates that the use of fluorescence lifetime not only improves the specificity of fluorescence measurements but also allows a more robust evaluation of data coll...Continue Reading

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Citations

Jun 15, 2006·Analytical Chemistry·Kristin A FletcherIsiah M Warner
Nov 19, 2005·Journal of Biomedical Optics·I MunroP M W French
Dec 22, 2005·Frontiers in Bioscience : a Journal and Virtual Library·William H YongLaura Marcu
Sep 2, 2008·Neurosurgical Focus·Sanjiv BhatiaWei-Chiang Lin
Jan 1, 2008·The Review of Scientific Instruments·P A A De BeuleP M W French
Jan 18, 2006·Journal of Biomedical Optics·Pramod V ButteLaura Marcu
Sep 18, 2007·Journal of Biomedical Optics·Jingjing WangLaura Marcu
May 13, 2010·Journal of Biomedical Optics·Pramod V ButteLaura Marcu
Nov 9, 2010·Journal of Biomedical Optics·Yinghua SunLaura Marcu
Jan 5, 2011·Journal of Biomedical Optics·Wei-Chiang LinJohn Ragheb
Jan 5, 2011·Journal of Biomedical Optics·Anthony KimBrian C Wilson
Nov 9, 2010·NeuroImage·Pramod V ButteLaura Marcu
Mar 2, 2016·Neurosurgical Focus·Pablo A ValdésAlexandra Golby
Jan 25, 2011·Biomedical Optics Express·William R LloydMary-Ann Mycek
Nov 1, 2018·Journal of Biomedical Optics·Dennis WirthKeith Paulsen
Jan 25, 2011·Biomedical Optics Express·James McGintyGordon W Stamp
Sep 18, 2017·Analytical and Bioanalytical Chemistry·Edwin OstertagRudolf W Kessler
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Dec 1, 2019·Journal of Photochemistry and Photobiology. B, Biology·Tatiana TozarRuxandra Pirvulescu
Jun 11, 2021·Frontiers in Oncology·Maria LukinaMarina Shirmanova

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Methods Mentioned

BETA
surgical resection
biopsy
fluorescence spectroscopy
laser-induced fluorescence spectroscopy
light microscopy

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