Fluorescence measurements of serotonin-induced V-ATPase-dependent pH changes at the luminal surface in salivary glands of the blowfly Calliphora vicina

The Journal of Experimental Biology
Julia ReinOtto Baumann

Abstract

Secretion in blowfly salivary glands is induced by the neurohormone serotonin and powered by a vacuolar-type H(+)-ATPase (V-ATPase) located in the apical membrane of the secretory cells. We have established a microfluorometric method for analysing pH changes at the luminal surface of the secretory epithelial cells by using the fluorescent dye 5-N-hexadecanoyl-aminofluorescein (HAF). After injection of HAF into the lumen of the tubular salivary gland, the fatty acyl chain of the dye molecule partitions into the outer leaflet of the plasma membrane and its pH-sensitive fluorescent moiety is exposed at the cell surface. Confocal imaging has confirmed that HAF distributes over the entire apical membrane of the secretory cells and remains restricted to this membrane domain. Ratiometric analysis of HAF fluorescence demonstrates that serotonin leads to a reversible dose-dependent acidification at the luminal surface. Inhibition by concanamycin A confirms that the serotonin-induced acidification at the luminal surface is due to H(+) transport across the apical membrane via V-ATPase. Measurements with pH-sensitive microelectrodes corroborate a serotonin-induced luminal acidification and demonstrate that luminal pH decreases by about 0.4...Continue Reading

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Citations

Apr 1, 2010·BMC Genomics·Juliana Alves-SilvaMichael J Lehane
Dec 3, 2011·Journal of Insect Physiology·Otto Baumann, Bernd Walz
Apr 15, 2008·Arthropod Structure & Development·Cathleen RotteOtto Baumann
Sep 24, 2015·Scientific Reports·Karolina JahnCarsten Hille
May 23, 2009·Archives of Insect Biochemistry and Physiology·Martin VossOtto Baumann
Mar 19, 2014·International Journal of Law and Psychiatry·Wendy Maycraft Kall
Jan 25, 2014·Nursing Forum·Susan H Lane, Reimund Serafica
Sep 18, 2007·The Journal of Biological Chemistry·Martin VossOtto Baumann
Nov 6, 2007·American Journal of Physiology. Cell Physiology·Julia ReinOtto Baumann
Feb 19, 2010·American Journal of Physiology. Cell Physiology·Martin VossOtto Baumann
Apr 18, 2018·Nature Ecology & Evolution·Christoph RatzkeJeff Gore
Mar 15, 2013·The Journal of Experimental Biology·Otto Baumann, Alexandra Bauer

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