PMID: 9439970Jan 24, 1998Paper

Fluorescence microscopy and three-dimensional imaging of the porcine corneal keratocyte network

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
C HahnelR Guthoff

Abstract

Little is known about the spatial arrangement and the corresponding morphometric data describing the living keratocyte network. For determination of alterations in corneal diseases it is crucial to know the morphology of the keratocyte network in the healthy state. Porcine cornea was used as a model tissue because it allows the study of species differences. Corneas from freshly enucleated pig eyes were stained with calcein AM and ethidium homodimer and examined by confocal laser scanning microscopy. High-resolution fluorescence images were used for three-dimensional reconstructions from which cell density and volume density were determined by computer-aided morphometry. Three keratocyte subpopulations were distinguished and visualized in their spatial arrangement. Significant differences with respect to both shape and fluorescence intensity distribution of the cell bodies were found. Cell volume density was 7.7% in the anterior stroma, 13.7% in the central stroma and 11.8% in the posterior stroma. The technique described allowed good visualization of the spatial arrangement of the keratocyte network. Combined with morphometric methods, the analysis of the state of the cornea yields a quantitative description. The method is expe...Continue Reading

References

May 1, 1991·Journal of Electron Microscopy Technique·S J Wright, G Schatten
Jun 1, 1995·Current Eye Research·T Møller-Pedersen, N Ehlers
Jul 1, 1993·Journal of the Optical Society of America. A, Optics and Image Science·C J KoesterR B Tackaberry
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Sep 1, 1955·American Journal of Ophthalmology·K SCHARENBERG

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Citations

Aug 9, 2003·Experimental Eye Research·Kurt MusselmannJohn R Hassell
May 29, 2010·Cornea·Gregor Wollensak, Hermann Herbst
Dec 10, 2002·Experimental Eye Research·Jeffrey K MarchantDavid E Birk

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