PMID: 6169004Jul 10, 1981Paper

Fluorescence modification of Escherichia coli 5S RNA

Nucleic Acids Research
M DigweedB Hardesty

Abstract

Reaction of 5S RNA with chlorocetaldehyde leads to the conversion of unpaired adenines to the fluorescent 1,N6-etheno-adenine derivatives. Up to 16 of the 23 adenines in free 5S RNA can be modified, the fastest reacting are A29, A34, A57-59. Partial modification of adenines in this area results in a 20% reduction in the efficiency of 5S RNA incorporation into 50S subunits during reconstitution and a 15% reduction in the activity of these subunits in peptide synthesis. Fluorescence from 1,N6-etheno-adenine is quenched in free 5S RNA and is not detectably further influenced by the binding of proteins E-L5, E-L18 and E-L25, nor by the first stage of the two step E. coli 50S subunit reconstitution procedure. However, the fluorescence is further reduced to near zero after the second step of the reconstitution. Thus, 5S RNS free in solution contains 16 unpaired adenines, those in the region between A29 and A59 particularly accessible to modification by chlorocetaldehyde. This portion of the 5S RNA molecule appears to undergo either a conformational change or interacts with other ribosomal components in the last stage of subunit reassembly.

References

May 16, 1977·European Journal of Biochemistry·H R Burrell, J Horowitz
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Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·I Larrinua, N Delihas
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Citations

Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T Pieler, V A Erdmann
Nov 25, 1982·Nucleic Acids Research·N Delihas, J Andersen
Dec 1, 1983·Journal of Biomolecular Structure & Dynamics·V A ErdmannT Pieler
Oct 1, 1982·European Journal of Biochemistry·M DigweedV A Erdmann
Jun 17, 2021·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·F Peter Guengerich, Pratibha P Ghodke

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