PMID: 8962467Nov 29, 1996Paper

Fluorescence of reduced nicotinamides using one- and two-photon excitation

Biophysical Chemistry
B KierdaszukJ R Lakowicz

Abstract

We examined the steady-state and time-resolved emission of NADH and NAMH resulting from one-photon and two-photon excitation. Similar emission spectra were observed for both modes of excitation. The fundamental anisotropy of NADH is near 0.54 for two-photon excitation from 690 to 740 nm, which is 46% higher than the value of 0.37 observed for one-photon excitation. This observation of a higher anisotropy with two-photon excitation was consistent with INDO/SDCI calculations of the one- and two-photon transitions. Minor differences in the multi-exponential decays of NADH were observed for one- and two-photon excitation, but presently available resolution does not allow us to conclude the decays are distinct. NADH-LADH-IBA complex formation led to an order of magnitude larger of the average lifetimes of NADH fluorescence resulting from one- and two-photon excitation. Fluorescence intensity and fluorescence anisotropy decays of NADH was double-exponential for both modes of excitation and show that the observed heterogeneity of the fluorescence decay kinetics of reduced nicotinamides arises from the inherent photoprocess of the dihydronicotinamide chromophore and not due to any intramolecular interactions with adenine part of NADH. ...Continue Reading

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J R LakowiczM L Johnson
Apr 6, 1990·Science·W DenkW W Webb
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R R BirgeK Nakanishi
Apr 14, 1987·Biochemical and Biophysical Research Communications·G KrishnamoorthyB Venkataraman
May 8, 1969·Biochemical and Biophysical Research Communications·D J Cannon, R H McKay
Jan 5, 1982·Biochemistry·M R Eftink, L A Selvidge
Nov 1, 1995·Biophysical Chemistry·J R LakowiczI Gryczynski
Jun 15, 1955·Proceedings of the National Academy of Sciences of the United States of America·B L Vallee, F L Hoch

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Citations

Sep 1, 1996·Journal of Fluorescence·I GryczynskiJ R Lakowicz
Feb 16, 2005·Journal of Fluorescence·K D BelfieldK J Schafer
Apr 23, 2003·Biosensors & Bioelectronics·Cory EstesLinda Powers
Aug 16, 2008·Biochemistry·Ksenia BlinovaRobert S Balaban
Apr 2, 2010·Chemical Reviews·Mikhail Y Berezin, Samuel Achilefu
Oct 11, 2008·Tissue Engineering. Part B, Reviews·Irene GeorgakoudiDavid L Kaplan
Oct 11, 2011·Tissue Engineering. Part C, Methods·Roman DittmarKeita Ito
Nov 10, 2001·Annual Review of Biomedical Engineering·P T SoK M Berland
Jan 1, 1997·Annual Review of Physical Chemistry·P R Callis
May 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·G H PattersonD W Piston
Sep 25, 1999·IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society·H Malak
Dec 15, 2004·Biophysical Journal·Emily C RothsteinRobert S Balaban
Apr 20, 2002·Biophysical Journal·Shaohui HuangWatt W Webb
Aug 16, 2016·Free Radical Biology & Medicine·Thomas S Blacker, Michael R Duchen
Sep 6, 2018·Journal of Biomedical Optics·Lydia SauerMartin Hammer
Feb 18, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Alexa von KettelerWolfgang Petrich
Sep 2, 2020·Journal of Fluorescence·Anastasiia A KozlovaDaniil N Bratashov
Jul 26, 2016·Biomedical Optics Express·Ning MaEnrico Gratton
Aug 24, 2006·American Journal of Physiology. Renal Physiology·Liping WuPhilip R Mayeux
Apr 26, 2019·The Journal of Physical Chemistry. B·Thomas S BlackerAngus J Bain
Jan 1, 2011·Chemistry of Materials : a Publication of the American Chemical Society·S Sumalekshmy, Christoph J Fahrni
Nov 12, 2020·The Journal of Physical Chemistry. B·Ioanna A GorbunovaOleg S Vasyutinskii

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