PMID: 6162881Jan 1, 1981Paper

Fluorescence of yeast vitally stained with ethidium bromide and propidium iodide

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
D A Corliss, W E White

Abstract

Both ethidium bromide and propidium iodide stain growing yeast. As visualized in the fluorescence microscope, ethidium stains the nucleus and cytoplasm in wild type yeast and in those grown in 10% dextrose, with brightly fluorescent cytoplasmic granules being present in both. Under the latter conditions, the mitochondria are repressed but not absent. In rho 0 cells, in which the mitochondrial DNA is absent, ethidium appears to bind to the cell wall or membrane preferentially with no cytoplasmic granules being visible. In all cell types, propidium appears to bind the cell wall or membrane with no cytoplasmic granules being visible in any cell. The staining patterns thus suggest greater differences in the binding of these two types to mitochondrial DNA in situ than is suggested by their in vitro behavior. These differences in binding could explain their different mutagenic capacities..

Citations

Apr 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H L Bank
Sep 1, 1995·The Journal of Cell Biology·S JonesN Segev
Sep 6, 2012·Molecular and Cellular Biology·Kathryn S DorisBrian A Morgan
Feb 3, 2005·Molecular and Cellular Biology·Félix Prado, Andrés Aguilera
Jul 20, 2000·Archives of Biochemistry and Biophysics·T Bernas, J W Dobrucki
Sep 10, 2015·Biomacromolecules·Ilya LetnikCharles Greenblatt
Jun 22, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Agustina PostigoMaximiliano Sortino
Nov 8, 2016·Aquaculture·Leticia Torres, Terrence R Tiersch
Jan 1, 1985·Journal of Basic Microbiology·D Kuchenbecker, G Braun
Nov 14, 2006·The Journal of Biological Chemistry·Su Jeoung SuhJulian I Schroeder
Apr 1, 1984·Journal of Clinical Microbiology·J D MansourT H Schulte
Mar 1, 1996·Molecular and Cellular Biology·M M SmithP C Megee

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