Fluorescence Polarization Anisotropy in Microdroplets

The Journal of Physical Chemistry Letters
Zhenpeng ZhouRichard N Zare

Abstract

Chemical reactions can be greatly accelerated in microdroplets, but the factors that lead to acceleration are still being elucidated. Using rhodamine 6G (R6G) as a model compound, we studied the density distribution and fluorescence polarization anisotropy of this dye in water-in-oil microdroplets. We found the density of R6G is higher on the surface of the microdroplets, and the ratio of the surface density to that of the center grows with increasing microdroplet radius or with decreasing R6G concentration. The measured fluorescence polarization anisotropy at the surface is almost the same for droplets of different sizes but becomes larger when the concentration is lowered. We also performed three-dimensional simulations by treating R6G+ and its associated anion as a dipole of fixed length and magnitude. The simulation results match quite well the experimental measurements, showing that the density distribution and fluorescence polarization anisotropy can be largely explained by a simple electrostatic model.

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Citations

Dec 15, 2019·Angewandte Chemie·Elumalai GnanamaniRichard N Zare
Jul 21, 2020·Journal of Mass Spectrometry : JMS·Yangjie LiR Graham Cooks
Feb 29, 2020·Chemical Science·Chengyuan LiuRichard N Zare
Feb 28, 2020·Nature Communications·Xiaoqin ZhongRichard N Zare
Apr 22, 2020·Annual Review of Physical Chemistry·Zhenwei WeiXin Yan
Nov 25, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jae Kyoo LeeRichard N Zare
Feb 17, 2021·Analytical Chemistry·Pengyi ZhaoHao Chen
Apr 21, 2021·Chemical Communications : Chem Comm·Dacheng KuaiXin Yan
Jun 9, 2021·Journal of the American Society for Mass Spectrometry·Tobias RainerThomas Müller
Feb 4, 2020·Chemical Science·Andrew C CavellRandall H Goldsmith
Aug 9, 2019·Journal of the American Society for Mass Spectrometry·Patrick W FedickR Graham Cooks
Aug 15, 2019·Journal of the American Society for Mass Spectrometry·Brett M MarshR Graham Cooks
Jun 15, 2021·Chemical Science·Kevin R WilsonMichael I Jacobs
May 27, 2020·The Journal of Physical Chemistry. a·Namita NarendraTillmann Kubis
Aug 18, 2020·The Journal of Physical Chemistry Letters·Hanqing XiongWei Min
Jun 28, 2019·Journal of the American Chemical Society·Jae Kyoo LeeRichard N Zare
Oct 22, 2020·The Journal of Physical Chemistry. B·Hanqing XiongWei Min

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