PMID: 1195335Apr 23, 1975Paper

Fluorescence polarization in a planar array of pigment molecules: theoretical treatment and application to flavins incorporated into artificial membranes

The Journal of Membrane Biology
E Frehland, H W Trissl

Abstract

A quantitative fluorescence polarization theory of molecules bound to two-dimensional plane layers has been developed when the electronic transition moments of absorption and emission are parallel within the fluorescent molecules. The transition moments are assumed to be in preferred orientation with respect to the normal to the plane and to be randomly oriented within the plane (rotational symmetry with the normal as axis of symmetry). Three basic model distributions of transition moments are investigated quantitatively. These model distributions represent a simplification but in most cases may be expected to describe reality with sufficient accuracy. For all distributions, two cases of different mobility of molecules are treated: (a) the lifetime of fluorescence is small compared with the characteristic relaxation time of the distribution, and (b) the lifetime of fluorescence is long, so that a complete reorientation of transition moments during the excited state can take place. From the quantitative calculations four characteristic quantities are derived, which are appropriate for the analysis of experimental data. Experiments are carried out with phosphatidylcholine bilayer membranes which contain three differently substitu...Continue Reading

References

Nov 15, 1974·Biochimica Et Biophysica Acta·H W Trissl
Jan 16, 1973·Biochemistry·R A BadleyH Schneider
Jun 23, 1972·Biochimica Et Biophysica Acta·R J CherryD Chapman
Oct 1, 1971·Biochemical and Biophysical Research Communications·R A BadleyW G Martin
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J Yguerabide, L Stryer
Jun 1, 1950·The Biochemical Journal·G WEBER
Dec 1, 1972·The Journal of Membrane Biology·A SteinemannP Läuger

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