Fluorescence Quenching Determination of Uranium (VI) Binding Properties by Two Functional Proteins: Acetylcholinesterase (AChE) and Vitellogenin (Vtg)

Journal of Fluorescence
Frédéric CoppinSandrine Frelon

Abstract

The interactions between uranium and two functional proteins (AChE and Vtg) were investigated using fluorescence quenching measurements. The combined use of a microplate spectrofluorometer and logarithmic additions of uranium into protein solutions allowed us to define the fluorescence quenching over a wide range of [U]/[Pi] ratios (from 1 to 3235) at physiologically relevant conditions of pH. Results showed that fluorescence from the two functional proteins was quenched by UO2 (2+). Stoichiometry reactions, fluorescence quenching mechanisms and complexing properties of proteins, i.e. binding constants and binding sites densities, were determined using classic fluorescence quenching methods and curve-fitting software (PROSECE). It was demonstrated that in our test conditions, the protein complexation by uranium could be simulated by two specific sites (L1 and L2). The obtained complexation constant values are log K1 = 5.7 (±1.0), log K2 = 4.9 (±1.1); L1 = 83 (±2), L2 = 2220 (±150) for U(VI) - Vtg and log K1 = 8.1 (±0.9), log K2 = 6.6 (±0.5), L1 = 115 (±16), L2 = 530 (±23) for U(VI)-AChE (Li is expressed in mol/mol of protein).

References

Jul 1, 1994·Molecular Reproduction and Development·S Grbac-IvankovicF W Sunderman
Oct 1, 1995·Molecular Reproduction and Development·F W SundermanJ Ozols
Mar 1, 1996·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M KomatsuS Hayashi
Jul 21, 1998·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·R N GuedesB A Dover
Jun 5, 2004·Marine Environmental Research·M L Martín-DíazT A DelValls
Jun 21, 2005·Chemical Research in Toxicology·Claude VidaudEric Quéméneur
Sep 26, 2006·Biochimie·Eric AnsoborloValérie Moulin
Mar 22, 2007·Environmental Toxicology and Chemistry·Sabrina BarilletAlain Devaux
Dec 25, 2007·Journal of Fluorescence·Shubha PandeyH M Chawla
Jun 5, 2008·Journal of Fluorescence·Elmas GökNuriye Akbay
Apr 3, 2009·Biochimie·Ramanna V RajeshRathanam Boopathy
Apr 14, 2010·Journal of Inorganic Biochemistry·Bo LiHarald Foerstendorf
Jul 5, 2013·Chemistry : a European Journal·Samir SafiChristophe Den Auwer
Sep 27, 2013·The Journal of Toxicological Sciences·Philippe LestaevelMohammed Taouis

❮ Previous
Next ❯

Citations

Mar 21, 2017·Metallomics : Integrated Biometal Science·Y Eb-LevadouxS Mounicou
Sep 5, 2017·Chemistry : a European Journal·Sandrine Sauge-MerleCatherine Berthomieu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved