Fluorescence quenching study on the interaction of ferroferric oxide nanoparticles with bovine serum albumin

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Changchun HaoRunguang Sun

Abstract

Fluorescence quenching was used to study the potential interaction mechanism of Bovine serum albumin (BSA) with either hydrophilic ferroferric oxide (Fe3O4) nanoparticles (NPs) or hydrophobic Fe3O4 NPs. The experimental results indicated the mechanism between BSA and hydrophilic Fe3O4 NPs was static quenching and the one between BSA and hydrophobic Fe3O4 NPs was dynamic process that was drove by Förster's resonance energy transfer (FRET). And the binding parameters for the interaction of BSA with either hydrophilic or hydrophobic Fe3O4 NPs were calculated by using the fluorescence quenching measurement. The binding constant (KA) values of hydrophilic Fe3O4 NPs were 8518.73±23.35 (at 298K), 1190.31±15.41 (at 306K) and 321.97±8.57 (at 313K), respectively. The thermodynamic analysis implied that the intermolecular forces between BSA and hydrophilic Fe3O4 NPs were Van der Waals interaction or hydrogen bond, because the values of ΔH and ΔS between them were negative. While the one of BSA and hydrophobic Fe3O4 NPs involved hydrophobic forces, owing to the positive ΔH and ΔS between them. But they were all enthalpy-driven and exothermic, since their ΔG values were all negative. Synchronous fluorescence spectroscopy suggested that the ...Continue Reading

Citations

Feb 11, 2020·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Jie BaiXiping Ma
Apr 3, 2020·Journal of Biomolecular Structure & Dynamics·Masoumeh MehrabiAnwarul Hasan
Aug 28, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Ashish TiwariJaspreet Kaur Randhawa
May 31, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Xiujuan ZhaoFeng Zhang
Apr 14, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Carin C S BatistaFernando C Giacomelli
Jan 28, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Rinea BarbirIvana Vinković Vrček
Apr 1, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Anita UmerskaAriane Boudier
Nov 4, 2021·Journal of Food Biochemistry·Yi-Ting WenLin-Jun Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved