PMID: 20101988Jan 28, 2010Paper

Fluorescence resonance energy transfer between gentamycin and water-soluble CdTe QDs

Guang pu xue yu guang pu fen xi = Guang pu
Jin-Gui LiShuang-Yang Ding

Abstract

The water-soluble CdTe quantum dots (QDs) were prepared by using mercaptopropionic acid (MPA) as stabilizer in the aqueous system. Fluorescence resonance energy transfer (FRET) system was constructed between gentamycin (acceptor) and water-soluble CdTe QDs (donor). The maximal emission wavelength was 690 nm, and the line width of the fluorescence spectrum was very narrow (with the full width at half-maximum about 10 nm) and symmetric. The transfer of resonance energy from the CdTe QDs to gentamycin (GT) resulted in the fluorescence quenching of the QDs, corresponding to the increase in the concentration of GT. Several factors that impacted the fluorescence spectra of the FRET system, such as the excitation wavelength (305-425 nm), pH(5.0-11.0), ions (0-0.1 mmol x L(-1) PBS; 0-0.5 mmol x L(-1) NaCl), time (1-120 min), temperature (5-50 degrees C), and concentration of GT (2-80 mg x L(-1)), were investigated and refined. The linear ranges of GT concentration were 2-20 mg x L(-1), r = 0.986 7. Fourier transform infrared spectroscopy (FTIR) and high-performance liquid chromatography (HPLC) were used for confirming the chemical construction and relative specificity, respectively. The results indicated that sulfur and oxygen atoms in...Continue Reading

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