Fluorescence spectroscopic characterization of salivary metabolites of oral cancer patients

Journal of Photochemistry and Photobiology. B, Biology
Manoharan YuvarajSingaravelu Ganesan

Abstract

A pilot study has been carried out using human saliva in differentiating the normal subjects from that of oral squamous cell carcinoma (OSCC) patients, using the autofluorescence spectroscopy at 405nm excitation. A markable difference in the spectral signatures between the saliva of normal subjects and that of oral cancer patients has been noticed. The possible reasons for the altered spectral signature may be due to the presence of endogenous porphyrin, NAD(P)H and FAD in the exfoliated cells from saliva. The elevated level of porphyrin in saliva of OSCC patients may be attributed to the disturbances in the amino acid degradation pathway and heme biosynthetic pathway, during the transformation of normal into malignant cells. The integrated area under the curve of fluorescence emission spectrum at 405nm excitation and also fluorescence excitation spectrum for 625nm emission were compared for the saliva of normal and oral cancer patients. The area under the curve for the emission spectrum provides 85.7% sensitivity and 93.3% specificity, where as the fluorescence excitation spectrum discriminates the same with 84.1% sensitivity and 93.2% specificity.

References

Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·N RamanujamR Richards-Kortum
Oct 12, 2000·Forensic Science International : Synergy·N S SoukosN Kollias
May 27, 2003·Lasers in Surgery and Medicine·Diana C G de VeldJan L N Roodenburg
Aug 30, 2003·Photochemistry and Photobiology·S MadhuriS Ganesan
Feb 28, 2004·Expert Reviews in Molecular Medicine·Josephine S Modica-Napolitano, Keshav K Singh
Jun 11, 2004·Journal of Biomedical Optics·Stavros G DemosRalph deVere White
Jun 15, 2005·Lasers in Surgery and Medicine·Hsin-Ming ChenChih-Yu Wang
Nov 18, 2005·Nitric Oxide : Biology and Chemistry·Ning MaShosuke Kawanishi
Feb 14, 2006·Journal of Biochemical and Biophysical Methods·Ewa PobozyMarek Trojanowicz
Apr 18, 2008·Journal of Cancer Research and Therapeutics·C Murali KrishnaK Maheedhar
Apr 25, 2009·Clinics in Laboratory Medicine·Lenora R BiglerWilliam P Dubinsky
Jun 18, 2009·Lasers in Surgery and Medicine·J L JayanthiNarayanan Subhash
Sep 4, 2009·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Daniela Pereira LimaAna Cláudia Okamoto
Oct 23, 2009·Photochemistry and Photobiology·Jeyasingh EbenezarSingaravelu Ganesan
Mar 20, 2010·Metabolomics : Official Journal of the Metabolomic Society·Masahiro SugimotoMasaru Tomita
Mar 9, 2011·Clinical Chemistry·Tina PfaffeChamindie Punyadeera
Mar 1, 1994·Journal of Fluorescence·K Koenig, H Schneckenburger

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Citations

Jul 6, 2014·PloS One·Taotao MuFandong Meng
Jan 5, 2016·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Bilal H MalikKristen C Maitland
Jul 4, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Qin-Si WanKun-He Zhang
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Xun Chen, Dongsheng Yu
Jun 2, 2020·Frontiers in Physiology·Zhaojun NieQiyin Fang

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