PMID: 9540220Apr 16, 1998Paper

Fluorescence spectroscopy and photochemistry of phytochromes A and B in wild-type, mutant and transgenic strains of Arabidopsis thaliana

Journal of Photochemistry and Photobiology. B, Biology
V SineshchekovGarry C Whitelam

Abstract

Phytochrome (P) was characterized in etiolated seedlings of wild-type, mutant and transgenic strains of Arabidopsis with the use of low-temperature (85 K) fluorescence spectroscopy and photochemistry. The position (lambda max) of the Pr emission spectrum, its intensity (F0) proportional to [P tot] and the extent of the Pr-->lumi-R phototransformation at 85 K (gamma 1) were shown to vary depending on the plant strains and tissues used, while the extent of the Pr-->Pfr transformation at 273 K (gamma 2) remained relatively constant. Depletion of phyA (fre1-1 in Nagatani et al., Plant Physiol. 102 (1993) 269-277, and fhy2-2 in Whitelam et al., Plant Cell 5 (1993) 757-768) resulted in a steep decrease of F0 to approximately equal to 10%. The phyB mutant (hy3-B064 in Reed et al., Plant Cell 5 (1993) 147-157) revealed a slight reduction (by approximately equal to 20%) of F0 while lambda max and gamma 1 remained practically unaffected. In phyAphyB mutuant no P emmission was observed. Overexpression of oat phyA (13k7 and 21k15 in Boylan and Quail, Proc. Natl. Acad. Sci. USA 88 (1991) 10806-10810) brought about an increase of F0 by two or three times, a shift of lambda max to 685 nm and an increase of gamma 1 to 0.3-0.4. On the contrary,...Continue Reading

References

Nov 1, 1994·Photochemistry and Photobiology·V SineshchekovE Hartmann
Apr 17, 1997·Nature·J HughesT Börner
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M T Boylan, P H Quail
Dec 1, 1991·The Plant Cell·D. E. SomersP. H. Quail

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Citations

May 3, 2001·Journal of Photochemistry and Photobiology. B, Biology·V SineshchekovC Gatz
Nov 20, 2013·Journal of Photochemistry and Photobiology. B, Biology·V SineshchekovM Wada
Dec 12, 2012·Photochemistry and Photobiology·Chen SongJörg Matysik
Oct 10, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Vitaly SineshchekovAndrás Viczián
Mar 14, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·V SineshchekovJ-I Kim
Dec 1, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Vitaly A SineshchekovPeter Nick
Jan 1, 2019·Functional Plant Biology : FPB·V Sineshchekov
Jul 11, 2009·The Journal of Physical Chemistry. B·Piero AltoèMarco Garavelli

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