Fluorescence spectroscopy as an efficient tool for staging the degree of liver fibrosis: an in vivo comparison with MRI

Scientific Reports
Shaiju S NazeerRamapurath S Jayasree

Abstract

The study utilizes autofluorescence spectroscopy (AFS) along with multivariate spectral analysis for differentiating various stages of hepatic fibrosis. AFS has recently emerged as an efficient tool for evaluating the variations in different endogenous flurophores. In this study, the potential of AFS for differentiating the stages of liver fibrosis is assessed and compared with the results of enzyme evaluation, histopathology and the most advanced diagnostic tool, MRI. Using a fiber optic probe, the emission profile of the flurophores such as flavin adenine dinucleotide (FAD), lipofuscin-like lipopigments (lipopigments), porphyrins and the variation in the total hemoglobin concentration are evaluated in vivo on liver fibrosis induced animal models adopting a minimally invasive technique. Significant difference (p < 0.05) in the level of these biomarkers was observed between different stages of liver fibrosis. Normal hepatic tissue could be distinguished from mild and moderate hepatic fibrosis with a sensitivity of 95 to 100% and specificity of 90 to 100% using multivariate spectral analysis. The results are favourable to consider this technique as a potential tool for diagnosing liver fibrosis at an early stage, which is monume...Continue Reading

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Citations

Jun 30, 2019·Journal of Biophotonics·Chandavalli R RaghushakerKrishna K Mahato
Mar 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna C CroceLaura G Di Pasqua
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May 18, 2020·Scientific Reports·João L LagartoRiccardo Cicchi
Jun 19, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alana Aragón-HerreraFrancisca Lago

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Methods Mentioned

BETA
fluorescence spectroscopy
biopsies
biopsy
PCA
nuclear magnetic resonance
a

Software Mentioned

PCA
Datamax
LDA
SPSS
FibroScan
MATLAB

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