Fluorescence staining of human ovarian cancer tissue following application of 5-aminolevulinic acid: fluorescence microscopy studies

Lasers in Surgery and Medicine
Martin LöningGereon Hüttmann

Abstract

Application of 5-aminolevulinic acid (ALA) for fluorescence-guided second-look laparoscopy has been shown to be a promising new procedure in the early diagnosis of ovarian carcinoma metastases. However, for assessing the reliability of this method, information on the microscopic distribution of protoporphyrin IX (PP IX) in the tissue is needed. Additionally, the selectivity of PP IX uptake is essential for a potential photodynamic therapy (PDT) of ovarian cancer metastases. Thirty-six patients with epithelial ovarian cancer and two patients suffering from fallopian tube carcinoma underwent a laparoscopic second-look procedure 5 hours after the application of ALA. In 17 patients 36 fluorescence-guided biopsies were taken from fluorescing and non-fluorescing tissues for further evaluation. Fluorescence microscopy and digital image processing were utilized to determine the presence of PP IX fluorescence. A specificity of 88% and a sensitivity of 100% with a negative predictive value of 100% and a positive predictive value of 91% were calculated for PP IX fluorescence on a microscopic level as marker for ovarian cancer metastases. On a microscopic scale, ALA-induced PP IX fluorescence is confined to ovarian cancer tumor tissue spar...Continue Reading

References

Jul 30, 1992·Journal of Photochemistry and Photobiology. B, Biology·J C Kennedy, R H Pottier
Aug 1, 1992·Gynecologic Oncology·C La VecchiaS Franceschi
Jan 1, 1996·The Journal of Urology·M KriegmairA Hofstetter
Nov 1, 1996·Journal of Photochemistry and Photobiology. B, Biology·R BaumgartnerC Roth
Jun 24, 1998·Journal of the National Cancer Institute·T J DoughertyQ Peng
Apr 14, 1999·CA: a Cancer Journal for Clinicians·S H LandisP A Wingo
Jun 2, 2001·Journal of Photochemistry and Photobiology. B, Biology·A LeunigR Baumgartner
Oct 2, 2001·CA: a Cancer Journal for Clinicians·R T GreenleeM Thun
Jan 5, 2002·International Journal of Cancer. Journal International Du Cancer·Christian S BetzAndreas Leunig
Jun 25, 2002·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·Rolf-Markus Szeimies, Michael Landthaler
Nov 14, 2002·European Urology·Marie-Ange D'HallewinFrançois Guillemin
Mar 28, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Clive J KeltyRoger Ackroyd
Sep 28, 2004·Journal of Biomedical Optics·Vijay Vaidyanathan, Theresa W Fossum

❮ Previous
Next ❯

Citations

Jun 22, 2010·Translational Oncology·Rebecca M WilliamsWarren R Zipfel
Nov 4, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Magali Zeisser-LabouèbeNorbert Lange
Apr 10, 2009·Photodiagnosis and Photodynamic Therapy·Manuel AscencioSerge Mordon
Oct 24, 2007·Gynécologie, obstétrique & fertilité·M AscencioS Mordon
Dec 1, 2007·Photodiagnosis and Photodynamic Therapy·Manuel AscencioSerge Mordon
Apr 17, 2008·Lasers in Surgery and Medicine·Sven Rainer KantelhardtAlf Giese
Nov 11, 2017·Future Oncology·Henri AzaïsSerge Mordon
Apr 5, 2017·Gynécologie, Obstétrique, Fertilité & Sénologie·H AzaïsP Collinet

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.