Fluorescence studies of exchangeable apolipoprotein-lipid interactions. Superficial association of apolipophorin III with lipoprotein surfaces.

The Journal of Biological Chemistry
D SahooR O Ryan


Apolipophorin III (apoLp-III) from the Sphinx moth, Manduca sexta, is an 18-kDa exchangeable apolipoprotein that reversibly associates with lipoprotein particles. In the absence of lipid, apoLp-III exists as an elongated bundle of five amphipathic alpha-helices. Upon lipid association, the protein is postulated to undergo a major conformational change, wherein the bundle opens around hinge loop regions, resulting in exposure of its hydrophobic interior. Fluorescence quenching techniques have been employed to study apoLp-III helix topography and spatial arrangement in phospholipid disc complexes and intact lipoprotein particles. Intrinsic fluorescence of the single tyrosine in apoLp-III was exploited to monitor the location of helix 5 in model disc complexes. To investigate other regions of the protein, site-directed mutagenesis was performed to introduce cysteine residues, replacing Asn-40 (helix 2, N40C) or Leu-90 (helix 3, L90C), thereby providing two mutant apoLp-IIIs, each with a single site for covalent attachment of the extrinsic fluorescent probe, N-(1-pyrene) maleimide. In the lipid-free state, pyrene-N40C- and pyrene-L90C-apoLp-III were highly accessible to the negatively charged aqueous quencher KI, yielding Ksv value...Continue Reading


Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 16, 1991·Biochemical and Biophysical Research Communications·H LiuR O Ryan
Jan 22, 1991·Biochemistry·D R BreiterH M Holden
Jan 1, 1990·Proteins·J P SegrestG M Anantharamaiah
Apr 17, 1990·Biochimica Et Biophysica Acta·R BrasseurM Rosseneu
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A G SuitsJ W Heinecke
Jun 12, 1985·Biochimica Et Biophysica Acta·E Blatt, W H Sawyer
Jul 1, 1981·Analytical Biochemistry·M R Eftink, C A Ghiron
Oct 1, 1994·Insect Biochemistry and Molecular Biology·B J Blacklock, R O Ryan
Jan 1, 1994·Advances in Protein Chemistry·J P SegrestG M Anantharamaiah
Oct 25, 1996·The Journal of Biological Chemistry·Vasanthy NarayanaswamiR O Ryan
Dec 15, 1996·European Journal of Biochemistry·C BenetolloM Rosseneu
Aug 1, 1997·Biochemical Society Transactions·V Narayanaswami, R O Ryan


Jul 30, 2002·Journal of Molecular Biology·D SahooVasanthy Narayanaswami
Jul 7, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C A FisherR O Ryan
Dec 22, 1999·Biochimica Et Biophysica Acta·Vasanthy Narayanaswami, R O Ryan
May 20, 2009·Biochimica Et Biophysica Acta·Lesley J VasquezPaul M M Weers
Feb 15, 2005·The Journal of Biological Chemistry·Jessica Drury, Vasanthy Narayanaswami
Dec 14, 2004·The Biochemical Journal·V RaussensVasanthy Narayanaswami
Jan 29, 2000·European Journal of Biochemistry·Vasanthy NarayanaswamiR O Ryan
Mar 28, 2003·The Journal of Biological Chemistry·Armelle Varnier AgasøsterAurora Martínez

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