PMID: 9549622Apr 29, 1998Paper

Fluorescent and radiolabeling of polysaccharides: binding and internalization experiments on vascular cells

Journal of Biomedical Materials Research
S Prigent-RichardD Letourneur

Abstract

Glycosaminoglycans (GAGs) such as heparan sulfates are complex carbohydrate polymers. These structural components of the extracellular matrix are essential for the adhesion, migration, and regulation of cellular growth. To understand the physiological role of GAGs and GAG analogues, a practical approach consists of labeling and detecting them in cell extracts, or analyzing binding domains and their distributions into the cells. We propose a convenient and reliable method for preparing and labeling amino-enriched, polysaccharides with the fluorescent derivative 5-[(4,6-dichlorotriazine-2-yl)amino]-fluorescein (DTAF). Radioiodination is then performed on the DTAF moiety. This method was applied to polysaccharides known to inhibit vascular smooth-muscle cell (SMC) proliferation such as functionalized dextrans derived from poly(alpha 1-6 glucose) and fucan, poly(L-fucose 4-sulfate) extracted from brown seaweed. Using autoradiography and confocal microscopy, we observed the fixation and internalization of labeled antiproliferative products in SMCs from rat aorta. These probes can be useful for the understanding of polysaccharide-cell interactions. In addition, the method presented here can be applied to various synthetic or natural ...Continue Reading

References

Jan 1, 1979·Thrombosis Research·J Dawes, D S Papper
Apr 30, 1992·Biochemical and Biophysical Research Communications·T A McCaffreyB B Weksler
Jul 1, 1990·Analytical Biochemistry·T Mehlman, W H Burgess
Jan 1, 1991·Journal of Biomaterials Science. Polymer Edition·T Avramoglou, J Jozefonvicz
Oct 15, 1991·Thrombosis Research·S ColliecJ Jozefonvicz
Jan 1, 1985·Biomaterials·M MauzacM D Kazatchkine
Jul 1, 1988·Journal of Cellular Physiology·C Biswas
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A K LarsenJ Folkman
Jan 1, 1987·Analytical Biochemistry·J W Smith, D J Knauer
Jul 1, 1985·Journal of Cellular Physiology·J J CastellotM J Karnovsky
Apr 15, 1983·Analytical Biochemistry·C G GlabeS D Rosen
Sep 7, 1995·Biochemical Pharmacology·J NeytsS Ikeda
Oct 1, 1995·Biomaterials·H ThomasM D Kazatchkine
Apr 1, 1996·Journal of Biomedical Materials Research·D LogeartP Kern

❮ Previous
Next ❯

Citations

Mar 4, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·D LetourneurM Cansell
Oct 21, 2003·Journal of Biomedical Materials Research. Part a·Claire PariselDidier Letourneur
Dec 7, 2010·Colloids and Surfaces. B, Biointerfaces·Qingrong WeiZhongwei Gu
May 22, 2002·Journal of Vascular Surgery·Jean-François DeuxDidier Letourneur
Jul 6, 2000·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·B Montdargent, D Letourneur
Jul 9, 1999·Journal of Biomedical Materials Research·M CansellD Letourneur
Mar 20, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S ShohamS M Levitz
Dec 21, 2016·Macromolecular Bioscience·Catarina OliveiraTiago H Silva
Jun 24, 2008·Bioconjugate Chemistry·Alan R KatritzkyTamari Narindoshvili
Nov 12, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Jie ZhaoJunbai Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.