Fluorescent androgen derivatives do not discriminate between androgen receptor-positive and -negative human tumor cell lines

The Prostate
E M BernsT F de Goeij


For the evaluation of histochemical procedures for detection of androgen receptors, three human tumor cell lines have been used: PC-93 and NHIK-3025, both biochemically characterized as androgen receptor-positive, and EB-33, biochemically characterized as androgen receptor-negative. The binding of three fluorescent ligands, testosterone-17 beta-hemisuccinate-bovine serum albumin-fluorescein isothiocyanate, testosterone-17 beta-hemisuccinate-fluoresceinamine, and 5 alpha-dihydrotestosterone-17 beta-hemisuccinate-fluoresceinamine, to the cells was evaluated. The relative binding affinities of the ligands for the androgen receptors were low (less than 5% when compared to methyltrienolone). Treatment of the cells with the androgen-fluoresceinamine derivatives resulted in a fluorescent labeling of the cytoplasm in both intact and "freeze-damaged" cells of the three cell lines. This staining was independent of the presence of receptors. Nuclei were not stained. Incubation of intact cels with the protein-linked conjugate did not result in significant cellular fluorescence. Only cells with damaged membranes showed a positive histochemical reaction, both in nucleus and cytoplasm, irrespective of the receptor content of the cells. The fl...Continue Reading


Sep 1, 1978·Molecular and Cellular Endocrinology·E MulderR Oftebro
Mar 1, 1983·Tissue Antigens·F H Claas, G J van Steenbrugge
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Aug 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L P PertschukK B Eisenberg

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