PMID: 41847Aug 1, 1979

Fluorescent antibody test kit for rapid detection and identification of members of the Bacteroides fragilis and Bacteroides melaninogenicus groups in clinical specimens

Journal of Clinical Microbiology
R W HollandW A Altemeier

Abstract

The Fluoretec fluorescent antibody test kit (Pfizer Inc., New York, N.Y.), developed for the rapid detection of members of the Bacteroides fragilis and B. melaninogenicus groups, was evaluated by testing 58 stock cultures and 76 clinical specimens. The test reagents detected 100% of 40 B. fragilis and B. thetaiotaomicron stock culture strains, although only 22% of 18 B. vulgatus, B. distasonis, and B. ovatus strains showed positive fluorescence. The 76 clinical specimens were evaluated by examining fluorescent antibody-stained smears of 49 specimens of purulent material and smears of 27 blood cultures which were positive for gram-negative bacilli by Gram stain or subculture. The fluoretec reagent detected members of the B. fragilis group in 28 (97%) of the 29 specimens of purulent material and all (100%) of the 16 blood cultures in which these anaerobes were demonstrated by culture. Overall, the Fluoretec reagent detected members of B. fragilis group in 44 (98%) of the 45 clinical specimens which were shown by culture to contain these anaerobes. Two of the 76 clinical specimens gave positive fluorescence for members of the B. fragilis group but failed to yield these organisms by culture. Members of the B. melaninogenicus group ...Continue Reading

Citations

Jan 1, 1981·Critical Reviews in Microbiology·R F D'AmatoE J Bottone
Aug 1, 1986·European Journal of Clinical Microbiology·A VegeF Meisel-Mikolajczyk
Feb 1, 1984·The Journal of Applied Bacteriology·K Allsop, D J Stickler
May 1, 1996·Letters in Applied Microbiology·T KuwaharaY Ohnishi
Feb 1, 1982·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·A SchwanU Forsum
Aug 1, 1980·Clinical Pediatrics·I BrookS M Finegold

Related Concepts

Anaerobiosis
Bacteroides
Bacteroides fragilis
Bacteroides Infections
Prevotella melaninogenica
Immunofluorescence Assay
Pus

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