Fluorescent bead arrays by means of layer-by-layer polyelectrolyte adsorption

Soft Matter
Andreas SchnäckelEdwin Donath

Abstract

Colloids with graduated fluorescence intensities were fabricated by means of layer-wise adsorption of fluorescein isothiocyanate-labelled poly(allyl amine hydrochloride) (FITC-PAH) together with poly(styrene sulfonate) (PSS) on silica particles. The graduated fluorescence was adjusted by variation of the fluorescent layer number and mixing labelled PAH with unlabelled PAH in one layer. The graduation of fluorescence intensities was adjusted in a geometric progression. It was shown that a proper label content is crucial if self-quenching phenomena are involved. The approach of mixing FITC-PAH with unlabelled polyelectrolyte during adsorption was unsatisfactory since competition in adsorption occurs. The system shows excellent stability at least over a period of two years.

References

Sep 15, 1999·Journal of Immunological Methods·R T Carson, D A Vignali
Feb 13, 2001·Journal of Colloid and Interface Science·Wenjun YangFrank Caruso
Mar 13, 2001·International Review of Cytology·G Boeck
Apr 20, 2001·Journal of Microencapsulation·B NeuH Bäumler
Jul 27, 2001·Journal of Medical Virology·D G Baetens, L M Van Renterghem
Feb 28, 2002·Journal of Immunological Methods·Sun W TamKrishnanand D Kumble
Feb 4, 2003·Chemistry : a European Journal·Edwin DonathHelmuth Möhwald
May 27, 2003·Clinical Chemistry·Attila TárnokRudi Varro
Mar 30, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Edward MorganAmitabh Gaur
Jul 13, 2004·Biomacromolecules·Sabine HillerEdwin Donath
Apr 14, 2005·Angewandte Chemie·Martin FischlechnerEdwin Donath
Dec 16, 2005·Angewandte Chemie·Martin FischlechnerEdwin Donath
Dec 31, 2005·Investigative Ophthalmology & Visual Science·Kenneth G-J OoiVirginia L Calder
Feb 4, 2006·Macromolecular Bioscience·Uta ReibetanzEdwin Donath
Feb 17, 2006·Methods : a Companion to Methods in Enzymology·Mohamed F Elshal, J Philip McCoy
Sep 4, 1998·Angewandte Chemie·Edwin DonathHelmuth Möhwald

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.