Fluorescent Bocillins: synthesis and application in the detection of penicillin-binding proteins

Electrophoresis
K R GeeL C Blaszcak

Abstract

Novel fluorescent analogs of penicillin V were synthesized and evaluated for efficacy in the detection of penicillin binding proteins (PBPs). These molecules include the full structure of penicillin V, with the potent Bodipy fluorophore attached to the para-position of the penicillin V phenyl group. The green fluorescent Bocillin FL and the near-infrared (IR) fluorescent Bocillin 650/665 probes were shown to bind to PBPs, both purified and from membrane preparations, with high affinity and specificity. These reagents allow for facile detection of 2-4 ng of purified PBP with the aid of a fluorescent scanner.

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Citations

Feb 6, 2002·Current Opinion in Chemical Biology·Wayne F Patton, Joseph M Beechem
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wayne F Patton
Jul 17, 2012·Applied and Environmental Microbiology·Zachary L PrattCharles W Kaspar
Aug 27, 2011·Analytical Biochemistry·Aleksey RukavishnikovSchuyler Corry
Jun 18, 2016·Nature Chemical Biology·Ozden Kocaoglu, Erin E Carlson
Mar 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shahla M Abdullah, Shwan Rachid
Feb 12, 2020·Antimicrobial Agents and Chemotherapy·Dušanka DjorićChristopher J Kristich
Oct 11, 2007·The Journal of Biological Chemistry·Xin FanDasantila Golemi-Kotra
Oct 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jonathan HiraChristian S Lentz
Jan 21, 2021·ACS Infectious Diseases·Samantha R Levine, Kimberly E Beatty
Jun 16, 2021·Bioorganic & Medicinal Chemistry·Phuong Luong, Danielle H Dube

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