PMID: 8972631Dec 1, 1996Paper

Fluorescent characteristics and pharmacokinetic profiles of the fluorescent probe BCECF in various tissues: the role of blood content

Photochemistry and Photobiology
J M DevoisselleH Maillols

Abstract

The purpose of this study was to investigate the in vitro and in vivo spectral characteristics of the fluorescent pH-sensitive probe bis-carboxyethylcarboxyfluorescein (BCECF) in different tissues and its fluorescence kinetics profiles. The in vivo study was performed on anesthetized adult Wistar rats. After intravenous administration (4.8 mg/kg), fluorescence spectra were recorded on the following tissues: skin, an isolated blood vessel and liver. Measurements performed in vitro on blood samples show modifications of the BCECF emission spectrum with a blue-shift (10 nm) and a low fluorescence emission. Blood content greatly influences the pH measurement by increasing the I(490 exc., 530 em.)/I(470 exc., 530 em.) Fluorescence ratio value (ratio of the fluorescence intensities at 530 nm following excitation at 470 nm and 490 nm) when the hematocrit is high. A 0.35 ratio difference is observed between a BCECF-buffered solution and blood samples of 44% hematocrit. The emission spectra recorded on the skin are quite similar to the emission spectrum of BCECF in aqueous solution and are consistent with an extravascular localization of the dye a few minutes after injection. On the contrary, spectra recorded on the blood vessel and the...Continue Reading

References

Nov 1, 1979·Pflügers Archiv : European journal of physiology·A BollingerF Mahler
Oct 1, 1978·Experimental Eye Research·F C DeloriR H Webb
May 15, 1992·Journal of Photochemistry and Photobiology. B, Biology·S MordonD Coustaud
May 1, 1991·Journal of Photochemistry and Photobiology. B, Biology·S MordonD Coustaut
Mar 1, 1990·Experimental Eye Research·J V ThomasR F Brubaker
Jan 1, 1989·Methods in Cell Biology·R Y Tsien
Jul 1, 1986·Analytical Biochemistry·M L GraberE Pastoriza-Munoz
Jan 1, 1985·Experimental Eye Research·D GrotteR Brubaker
Jul 1, 1995·Journal of Photochemistry and Photobiology. B, Biology·D A RussellD P Valenzeno
Mar 1, 1994·Photochemistry and Photobiology·D A RussellD P Valenzeno

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