Fluorescent coelenteramide-containing protein as a color bioindicator for low-dose radiation effects

Analytical and Bioanalytical Chemistry
Alena S PetrovaN S Kudryasheva

Abstract

The study addresses the application of fluorescent coelenteramide-containing proteins as color bioindicators for radiotoxicity evaluation. Biological effects of chronic low-dose radiation are under investigation. Tritiated water (200 MBq/L) was used as a model source of low-intensive ionizing radiation of beta type. 'Discharged obelin,' product of bioluminescent reaction of marine coelenterate Obelia longissimi, was used as a representative of the coelenteramide-containing proteins. Coelenteramide, fluorophore of discharged obelin, is a photochemically active molecule; it produces fluorescence forms of different color. Contributions of 'violet' and 'blue-green' forms to the visible fluorescence serve as tested parameters. The contributions depend on the coelenteramide's microenvironment in the protein, and, hence, evaluate distractive ability and toxicity of radiation. The protein samples were exposed to beta radiation for 18 days, and maximal dose accumulated by the samples was 0.28 Gy, being close to a tentative limit of a low-dose interval. Increase of relative contribution of 'violet' fluorescence under exposure to the beta irradiation was revealed. High sensitivity of the protein-based test system to low-dose ionizing radi...Continue Reading

References

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Citations

Aug 1, 2018·Analytical and Bioanalytical Chemistry·Alena S PetrovaNadezhda S Kudryasheva
Sep 13, 2019·International Journal of Molecular Sciences·Nadezhda S Kudryasheva, Ekaterina S Kovel
Sep 15, 2020·Dose-response : a Publication of International Hormesis Society·Cuihua LiuBing Wang
Nov 15, 2020·International Journal of Molecular Sciences·Tatiana V RozhkoNadezhda S Kudryasheva
Mar 11, 2018·Biochimica Et Biophysica Acta. General Subjects·Om P MishraMelpo Christofidou-Solomidou
Jul 3, 2021·International Journal of Molecular Sciences·Cuihua LiuBing Wang
Sep 15, 2021·The Journal of Physical Chemistry. B·Shu-Feng ChenYa-Jun Liu

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