PMID: 7543914Sep 1, 1995Paper

Fluorescent conjugates of brefeldin A selectively stain the endoplasmic reticulum and Golgi complex of living cells

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Y DengJ W Yewdell

Abstract

The fungal metabolite brefeldin A (BFA) interferes with vesicular trafficking in most animal cells. To gain insight into the mechanism of BFA action, we esterified it to the fluorophore, boron dipyromethene difluoride (BODIPY). BODIPY-BEA localized predominantly in the endoplasmic reticulum (ER) and Golgi complex of viable cells and was extracted by detergent treatment, suggesting it interacts primarily with lipid bilayers. The localization of the conjugate is conferred by BFA, since free BODIPY or BODIPY esterified to cyclopentanol did not specifically localize to internal membranes. BODIPY-BFA exhibited a similar biological activity to BFA, but only when used at higher concentrations and after a delay. HPLC analysis revealed that over this period, cells converted BODIPY-BFA to species co-eluting with free BODIPY and BFA. Therefore, BODIPY-BFA is probably inactive until BFA is released by cellular esterases. The specific localization of BODIPY-BFA to the ER and Golgi complex suggests that BFA might exert its effects on vesicular trafficking by perturbing the lipid bilayer of its target organelles. Because BODIPY-BFA intensely stains the ER at concentrations that have no discernible effects on intracellular transport or other c...Continue Reading

Citations

Aug 22, 2007·Journal of Biomedical Science·Xiwen XiongXiang Gao
Aug 17, 1999·The Journal of Experimental Medicine·N Thieblemont, S D Wright
Dec 11, 2008·Reproductive Biology and Endocrinology : RB&E·Mauricio Díaz-MuñozVerónica Morales-Tlalpan
Apr 2, 2014·Cell Biology and Toxicology·Kentaro Oh-hashiKazutoshi Kiuchi
Aug 28, 1999·Neuropharmacology·B David-WatineP Bregestovski
May 23, 2013·Journal of Neurochemistry·Kumud KunjilwarPaul J Pfaffinger
Feb 24, 2009·Biochimica Et Biophysica Acta·Shaka S ScarlettJuergen F Kolb
Jan 19, 2006·Molecular Pharmacology·Jozsef LazarJeewoo Lee
Jan 7, 2003·The Journal of Biological Chemistry·Kentaro Oh-hashiMasaki Tanaka
Dec 17, 2008·Molecular and Cellular Biochemistry·Carlos SaldañaVerónica Morales-Tlalpan
Aug 13, 2008·Molecular and Cellular Biochemistry·Yingli ZhongJian Zhang
Aug 16, 2006·The Journal of Cell Biology·Fabien Vanden AbeeleNatalia Prevarskaya
Nov 15, 2018·ACS Chemical Biology·Raphael I BenhamouMicha Fridman

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