Fluorescent CXCL12AF647 as a novel probe for nonradioactive CXCL12/CXCR4 cellular interaction studies

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Sigrid HatseDominique Schols

Abstract

Chemokines drive the migration of leukocytes via interaction with specific G protein-coupled 7-transmembrane receptors. The chemokine ligand/receptor pair stromal cell-derived factor-1 (SDF-1, CXCL12)/CXCR4 is gaining increasing interest because of its involvement in the metastasis of several types of cancer and in certain inflammatory autoimmune disorders such as rheumatoid arthritis. In addition, CXCR4 serves as an important coreceptor for cellular entry of T-tropic strains of human immunodeficiency virus (HIV). Therefore, potent and specific CXCR4 antagonists may have therapeutic potential as anti-HIV, anti-cancer, and anti-inflammatory drugs. Chemokine receptor antagonists can be identified by their ability to inhibit ligand binding to the receptor protein. Until now, chemokine binding assays were mostly performed with radiolabeled chemokine ligands such as [(125)I]CXCL12. To overcome the practical problems associated with such radioactive chemokine binding assays, we have developed a flow cytometric technique using a new, commercially available Alexa Fluor 647 conjugate of CXCL12 (CXCL12(AF647)). Calcium flux, chemotaxis, and p44/42 mitogen-activated protein kinase phosphorylation assays showed that the agonistic activity ...Continue Reading

References

Feb 17, 1997·The Journal of Experimental Medicine·R I ConnorN R Landau
Oct 23, 1997·The Journal of Experimental Medicine·D ScholsE De Clercq
Oct 23, 1997·The Journal of Experimental Medicine·T MurakamiT Nagasawa
Jan 14, 1998·Nature Medicine·G A DonzellaJ P Moore
Apr 20, 1999·The Journal of Cell Biology·A V AndjelkovicJ S Pachter
Jun 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J A GonzaloJ C Gutierrez-Ramos
Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·T NankiP E Lipsky
Mar 29, 2001·Nature Immunology·M Thelen
Sep 5, 2001·Journal of Virology·F BaribaudR W Doms
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·P MatthysD Schols
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H GeminderA Ben-Baruch
Apr 12, 2002·The American Journal of Pathology·Nicholas W LukacsGary J Bridger
Sep 11, 2002·FEBS Letters·Sigrid HatseDominique Schols
Oct 22, 2002·The Journal of Biological Chemistry·Yan ZhouV Wee Yong
Dec 25, 2002·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Katrien PrincenDominique Schols
Jul 15, 2003·Journal of Biomolecular Screening·Anne-Christine ThierryStéphane Demotz
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Joshua B RubinRosalind A Segal
Nov 19, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Judith E BerlierRichard P Haugland

❮ Previous
Next ❯

Citations

May 17, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Preeti MisraJohn V Frangioni
May 11, 2012·Future Medicinal Chemistry·Ryo MasudaNobutaka Fujii
Apr 21, 2012·Organic & Biomolecular Chemistry·Shinya Oishi, Nobutaka Fujii
Jun 30, 2012·Chemical Society Reviews·Joeri KuilFijs W B van Leeuwen
May 17, 2015·Neuropharmacology·Leigh A StoddartStephen J Hill
Sep 1, 2015·British Journal of Pharmacology·Leigh A StoddartKevin D G Pfleger
Nov 13, 2010·Journal of Cellular and Molecular Medicine·David H McDermottPhilip M Murphy
Aug 1, 2006·Biochemical and Biophysical Research Communications·Sigrid HatseSandra Liekens
Aug 30, 2005·Current Opinion in Chemical Biology·Richard J Middleton, Barrie Kellam
Sep 24, 2013·Biochimica Et Biophysica Acta·Rajashri SridharanMark E Dumont
Jul 14, 2005·Biochemical Pharmacology·Sigrid HatseDominique Schols
Sep 5, 2015·Organic & Biomolecular Chemistry·Sunil HamalThomas W Bell
Jul 15, 2009·BioTechniques·Kathryn LukerGary Luker
Nov 28, 2013·Blood·Frédéric TorossianMarie-Caroline Le Bousse-Kerdilès
Oct 27, 2010·Journal of Biomolecular Screening·Jurriaan M ZwierEric Trinquet
Aug 16, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Siyu ZhuZiwei Huang
Jan 29, 2021·The FEBS Journal·Mark SoaveStephen J Hill

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.