PMID: 45395Feb 1, 1979

Fluorescent derivatives of nucleotides. Metal ion interactions and pH dependency

Biophysical Journal
J M VanderkooiG Woodrow

Abstract

The fluorescence parameters of ethenoadenosine derivatives are influenced by metal cations and pH, as summarized here. The pH profile of ethenoadenosine determined by fluorescence intensity gives a normal titration curve and is not affected by ionic strength. In contrast, the pH titration curves of etheno-ATP, etheno ADP, and etheno AMP depend upon ionic strength. At high ionic strength normal curves are obtained, whereas at low ionic strength anomalies are obtained; this suggests that the phosphates can interact with the ring, possibly by hydrogen binding to the ring nitrogens. The room temperature fluorescence of ethenoadenosine occurs from the base form, although excitation of either the acid or base forms can contribute to the emission. This result can be explained if the excited state pK is lower than the ground state pK, and if deprotonation occurs within the time scale of the excited state. At low pH values the fluorescence lifetime of the base form is dependent upon the buffer concentration, indicating that the reverse reaction, protonation, occurs. The affinity constants for the binding of metals to the ethenoadenosine phosphates resemble those for the corresponding adenosine phosphates. Ni(II) and Co(II) are more effe...Continue Reading

References

Jan 1, 1984·CRC Critical Reviews in Biochemistry·N J Leonard
Jan 30, 2018·Nucleosides, Nucleotides & Nucleic Acids·Alicja Stachelska-WierzchowskaBeata Wielgus-Kutrowska

Related Concepts

Energy Transfer
Derivatives
etheno-AMP
Metals
Fluorescence Spectroscopy
Fluorescent stain
Cations
Nucleotides
Fluorochromes
PH Profile Measurement

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