Fluorescent dyes for cell viability: an application on prefixed conditions

Histochemistry and Cell Biology
C FoglieniA M Davalli

Abstract

In recent years increasing attention has been given to apoptosis for its role in pathologic, organogenetic and homeostatic phenomena. Acridine orange (AO), Hoechst 33342 (HO) and propidium iodide (PI) are among the most used fluorescent dyes used to analyse cell culture viability. In fact, they respectively show specificity for living, apoptotic and late apoptosis/necrosis states. We explored whether HO, AO and PI can be used on prefixed monolayers of three commonly used cell lines. Here we mainly describe the metachromatic effects obtained by fluorescence microscopy with double and triple dye combinations. Furthermore, we propose an easy staining method in which a balanced sequential treatment with HO, AO and PI allows identification of different viability states onto fixed cells by using a long-pass FITC filter. This method extends the spectrum of suitable applications for these dyes in fluorescence viability detection onto previously fixed (prefixed) samples.

Citations

Sep 8, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Rafael NunezBeverly E Barton
Feb 16, 2008·American Journal of Respiratory Cell and Molecular Biology·Vadim ShlyonskyRobert Naeije
Feb 29, 2008·The Journal of Pharmacology and Experimental Therapeutics·Amina ArabChristoph Hehrlein
Jun 22, 2012·Journal of Fluorescence·Leo L ChanNing Lai
May 23, 2003·Journal of Biomedical Materials Research. Part a·S N SilvaM F Leite
Sep 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Helen L MaddockDerek M Yellon
Dec 14, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Biserka RelićMichel G Malaise
Nov 16, 2012·Analytical and Bioanalytical Chemistry·Katia WehbeGianfelice Cinque
Sep 5, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Narumol Bhummaphan, Pithi Chanvorachote
Feb 3, 2004·Free Radical Research·Ozgur Kutuk, Huveyda Basaga
Apr 16, 2013·Food & Function·Kotamballi N Chidambara MurthyBhimanagouda S Patil
Aug 3, 2004·Biology of Reproduction·George A ThouasGayle M Jones
Mar 1, 2007·Transplant International : Official Journal of the European Society for Organ Transplantation·Nils A 't HartRutger J Ploeg
Oct 21, 2010·Journal of Industrial Microbiology & Biotechnology·Leo L ChanBo Lin
Oct 3, 2003·The Journal of Clinical Investigation·Cyrus J ParsaWalter J Koch
Sep 30, 2014·Chinese Medical Sciences Journal = Chung-kuo I Hsüeh K'o Hsüeh Tsa Chih·Ling LiWen-li Xie
Jan 24, 2013·PloS One·Letícia Christina Pires GonçalvesErick Leite Bastos
Jul 1, 2006·Functional Plant Biology : FPB·Anna ZuppiniBarbara Baldan
Nov 3, 2007·Reproduction in Domestic Animals = Zuchthygiene·J SaragustyA Arav
Nov 16, 2001·Antimicrobial Agents and Chemotherapy·S W KamauA B Hehl
Jul 26, 2011·Nutrition Journal·Chiara FoglieniMaria Elena Ferrero
Feb 21, 2018·World Journal of Microbiology & Biotechnology·A P Isaac-MárquezC M Lezama-Dávila
Aug 7, 2019·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada· ShanmugapriyaSreenivasan Sasidharan
Jun 2, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·A Reenaa Joys AndasIsmail Adam Arbab
Aug 31, 2001·Biochemical and Biophysical Research Communications·G Ghosh ChoudhuryH E Abboud
Jan 13, 2006·Journal of the American Chemical Society·Christian R GoldsmithStephen J Lippard

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis