Fluorescent false neurotransmitter reveals functionally silent dopamine vesicle clusters in the striatum

Nature Neuroscience
Daniela B PereiraDavid Sulzer

Abstract

Neurotransmission at dopaminergic synapses has been studied with techniques that provide high temporal resolution, but cannot resolve individual synapses. To elucidate the spatial dynamics and heterogeneity of individual dopamine boutons, we developed fluorescent false neurotransmitter 200 (FFN200), a vesicular monoamine transporter 2 (VMAT2) substrate that selectively traces monoamine exocytosis in both neuronal cell culture and brain tissue. By monitoring electrically evoked Ca(2+) transients with GCaMP3 and FFN200 release simultaneously, we found that only a small fraction of dopamine boutons that exhibited Ca(2+) influx engaged in exocytosis, a result confirmed with activity-dependent loading of the endocytic probe FM1-43. Thus, only a low fraction of striatal dopamine axonal sites with uptake-competent VMAT2 vesicles are capable of transmitter release. This is consistent with the presence of functionally 'silent' dopamine vesicle clusters and represents, to the best of our knowledge, the first report suggestive of presynaptically silent neuromodulatory synapses.

References

Jan 1, 1977·Pharmacology & Therapeutics. Part B: General & Systematic Pharmacology·K FuxeU Ungerstedt
Aug 1, 1992·Journal of Neurochemistry·R T KennedyR M Wightman
Nov 1, 1974·The Journal of Cell Biology·S S Jahromi, H L Atwood
Jun 27, 2001·Nature Neuroscience·S S ZakharenkoS A Siegelbaum
Jul 19, 2002·Journal of Neurochemistry·Natsuki MatsushitaKazuto Kobayashi
Nov 5, 2003·Nature Biotechnology·Warren R ZipfelWatt W Webb
Jan 22, 2004·Proceedings of the National Academy of Sciences of the United States of America·Attila LosonczyZoltan Nusser
May 12, 2004·Neuron·Krista L MoulderSteven Mennerick
May 18, 2004·Nature Neuroscience·Hui Zhang, David Sulzer
May 18, 2004·Nature Neuroscience·Margaret E Rice, Stephanie J Cragg
Aug 30, 2005·Journal of Pharmaceutical and Biomedical Analysis·Mei-Yi Zhang, Chad E Beyer
Jun 16, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Krista L MoulderSteven Mennerick
Jul 26, 2006·Genesis : the Journal of Genetics and Development·Cristina M BäckmanAndreas C Tomac
Dec 19, 2006·Trends in Neurosciences·Gordon W Arbuthnott, Jeff Wickens
Jul 27, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·W Michael CaudleGary W Miller
Feb 22, 2008·American Journal of Physiology. Cell Physiology·Yoav AdamShimon Schuldiner
Jan 16, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Wakoto MatsudaTakeshi Kaneko
Apr 25, 2009·Alcoholism, Clinical and Experimental Research·Donita L RobinsonR Mark Wightman
Jun 13, 2009·Frontiers in Neural Circuits·Meera Mani, Timothy A Ryan
Nov 20, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·James A DanielBryce Vissel
Feb 5, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xiaoping JiangKrista L Moulder
Jun 11, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bibiana OnoaRobert H Edwards
Apr 14, 2011·The Journal of Physiology·Michael A Cousin, Gareth J O Evans
Sep 14, 2011·Methods in Molecular Biology·Minerva Y WongNigel S Bamford
Oct 1, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Fabio CacciapagliaRegina M Carelli

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Citations

Jul 30, 2019·Schizophrenia Bulletin·Seth H Walters, Edwin S Levitan
Jul 16, 2020·Movement Disorders : Official Journal of the Movement Disorder Society·Iara Pérez-TaboadaMario Vallejo
Sep 28, 2018·Nature Neuroscience·Cody A SicilianoKay M Tye
Jun 27, 2019·Journal of Vacuum Science & Technology. A, Vacuum, Surfaces, and Films : an Official Journal of the American Vacuum Society·Abraham G BeyeneMarkita P Landry
Oct 14, 2017·Brain Structure & Function·Marc Dos SantosNicolas Heck
May 21, 2019·Nano Research·Jackson Travis Del Bonis-O'DonnellMarkita P Landry
Aug 30, 2018·NPJ Parkinson's Disease·Stefano CataldiMatthew J Farrer
Nov 1, 2020·International Journal of Molecular Sciences·Marie A LabouesseTommaso Patriarchi
Dec 6, 2020·Pharmacology, Biochemistry, and Behavior·Alyssa G Yocky, Dan P Covey
Dec 5, 2020·Proceedings of the National Academy of Sciences of the United States of America·Mahalakshmi SomayajiDavid Sulzer
Dec 16, 2020·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jennifer E ZachryErin S Calipari
Nov 17, 2020·Frontiers in Molecular Neuroscience·Francesco Gobbo, Antonino Cattaneo
Mar 7, 2021·Micromachines·Chao TanXinyan Tracy Cui
Feb 16, 2019·Current Opinion in Neurobiology·Changliang Liu, Pascal S Kaeser
Apr 11, 2021·Nature Reviews. Neuroscience·Changliang LiuPascal S Kaeser
Apr 25, 2021·Cell Chemical Biology·Michael R Post, David Sulzer
Dec 31, 2020·Chemical Research in Toxicology·Carlie A BlackGary W Miller
Jul 3, 2021·Analytical Chemistry·Mutsuo NuriyaMasato Yasui
Jul 29, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Charles DucrotLouis-Eric Trudeau
May 13, 2020·Journal of the American Chemical Society·Tomas FialaDalibor Sames
Aug 11, 2017·ACS Chemical Neuroscience·Anand Kant DasSudipta Maiti
Dec 12, 2017·ACS Chemical Neuroscience·Kallol BeraSudipta Maiti
Jan 21, 2017·Analytical Chemistry·Mallikarjunarao GanesanaB Jill Venton
Aug 22, 2021·Journal of Neuroscience Methods·Jackson Travis Del Bonis-O'DonnellMarkita P Landry
Nov 9, 2021·Journal of Neuroscience Research·Rosaria FormisanoHarbinder S Dhillon

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Methods Mentioned

BETA
electron microscopy
transfection
nuclear magnetic resonance
NMR
Fluorescence
PCR
transgenic

Software Mentioned

AxioVision
StackReg
xy
MetaMorph
PoorMan3DReg
IGOR
GraphPad Prism
IGOR Pro
Puncta
MacBiophotonics ImageJ

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