Fluorescent immunosensor based on CuS nanoparticles for sensitive detection of cancer biomarker

The Analyst
Ying-Di ZhuJun-Jie Zhu

Abstract

A novel fluorescent immunosensor was developed based on the use of CuS nanoparticles (CuS NPs) as labels for the highly sensitive detection of human prostate cancer biomarker prostate specific antigen (PSA). In the presence of CuS NPs, the non-fluorescent substrate o-phenylenediamine could be oxidized into the stable fluorescent product 2,3-diamiophenazine at physiological pH. Throughout the reaction, no other oxidizing agents (e.g. hydrogen peroxide) were needed. The relatively mild oxidation conditions made the immunoassay robust, reliable and facile. The proposed immunoassay exhibited high sensitivity and specificity for the detection of PSA. A linear relationship between the fluorescent signals and the concentration of PSA was obtained in the range of 0.5 pg mL(-1) to 50 ng mL(-1), with a detection limit of 0.1 pg mL(-1) (S/N = 3). The proposed fluorescent immunoassay can be used as a promising platform for the detection of a variety of other biomarkers.

References

Dec 6, 2001·Annals of Clinical Biochemistry·A M WardF C Hamdy
Dec 2, 2004·Analytical Chemistry·Guodong LiuGreg E Collins
Apr 14, 2005·Chemical Reviews·Clemens BurdaMostafa A El-Sayed
Oct 22, 2005·Nature Reviews. Cancer·Joseph A Ludwig, John N Weinstein
Jun 21, 2007·Analytical Chemistry·Adriano AmbrosiArben Merkoçi
Oct 12, 2007·Analytical Chemistry·Rongjing CuiHong-Yuan Chen
Jul 26, 2008·Nature Nanotechnology·Lizeng GaoXiyun Yan
Jan 9, 2009·Angewandte Chemie·Atul AsatiJ Manuel Perez
Nov 27, 2009·Nature·David A Giljohann, Chad A Mirkin
Feb 22, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Ke ZhangXiaochun Wu
May 13, 2011·Chemical Communications : Chem Comm·Wenbing ShiYuming Huang
Jul 29, 2011·Chemical Society Reviews·Maëlle PerfézouArben Merkoçi
Jul 30, 2011·Advanced Materials·Yujun SongXiaogang Qu
Jan 10, 2012·Analytical Chemistry·Vladimir GubalaDavid E Williams
Jan 24, 2012·Chemical Communications : Chem Comm·Taihua LiMin-Gon Kim
Jan 1, 2010·Sensors·Young-Eun ChoiJoon Won Park
Jan 8, 2013·Journal of Agricultural and Food Chemistry·Xiaodan ZhangYuming Huang
Jun 29, 2013·Analytical Chemistry·Zhuangqiang GaoDianping Tang
Jul 23, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Lijing ZhangTengfeng Xie

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Citations

Nov 4, 2015·Analytical Methods : Advancing Methods and Applications·Christine F Woolley, Mark A Hayes
Jul 25, 2017·Analytical Chemistry·Ru-Jia YuYi-Tao Long
Jul 21, 2017·Methods and Applications in Fluorescence·Jian LvYi-Tao Long
Nov 22, 2018·Chemical Communications : Chem Comm·Yihong ZhongZhanjun Yang
May 8, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xiu WangPiaopiao Chen
Apr 13, 2017·Journal of Fluorescence·Shalini Menon, K Girish Kumar
Nov 24, 2016·Nanobiomedicine·Alejandro Chamorro-Garcia, Arben Merkoçi
Nov 6, 2018·Biosensors & Bioelectronics·Saif Mohammad Ishraq BariGergana G Nestorova

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