Fluorescent immunosorbent assay for the detection of alpha lactalbumin in dairy products with monoclonal antibody bioconjugated with CdSe/ZnS quantum dots

Food Chemistry
Anshu YangFenfen Cheng

Abstract

In this work, a new method termed competitive fluorescence-linked immunosorbent assay (FLISA) was developed for specifically quantification of bovine α-lactalbumin (α-La) in dairy products. The monoclonal antibodies (mAbs) against α-La were produced through hybridoma technology, and the mAbs were covalently conjugated with the CdSe/ZnS quantum dots (QDs) using the crossing-linking reagents. Moreover, a competitive FLISA based on QD-mAb conjugates was established to detect α-La in dairy products. It was shown that there was a good linear relationship between inhibition efficiency, and logarithm of α-La concentration after the detection parameters were optimised in which the concentration of α-La varied from 0.1 to 1000ng/mL. The value of IC50 was 0.03μg/mL, and the FLISA method exhibited high sensitivity with the LOD at 0.1ng/mL. The developed FLISA has been successfully applied to determine α-La in commercial dairy products, providing more sensitive analysis compared with the ELISA method.

References

Aug 24, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A CarroccioG Iacono
Apr 3, 2004·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Frank Pessler, Morris Nejat
Sep 1, 2005·Luminescence : the Journal of Biological and Chemical Luminescence·Qidan ChenQinhan Jin
Jun 23, 2006·Luminescence : the Journal of Biological and Chemical Luminescence·Xinyan WangXingguang Su
Aug 15, 2006·Biochemical and Biophysical Research Communications·William W YuVicki L Colvin
May 4, 2007·Journal of Agricultural and Food Chemistry·Hubert ChassaigneArjon J van Hengel
Dec 25, 2007·The Korean journal of laboratory medicine·Ki Hong KimDong Cheul Moon
May 23, 2008·Advanced Drug Delivery Reviews·Andrew M SmithShuming Nie
Aug 9, 2008·International Journal of Nanomedicine·Igor L MedintzAaron R Clapp
Jan 22, 2011·Toxicology Letters·Betty C A M van EschLéon M J Knippels
Jun 8, 2011·Analytical Biochemistry·Wael MahmoudAlyona Sukhanova
Feb 7, 2012·Journal of Agricultural and Food Chemistry·Jung-Feng Hsieh, Pei-Hsin Pan
Jun 20, 2012·Journal of the American Society for Mass Spectrometry·Timothy R CroleySteven M Musser

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Citations

Feb 15, 2016·Current Allergy and Asthma Reports·Julia ZimmerSusanne Kaul
Apr 14, 2016·Critical Reviews in Analytical Chemistry·Jyoti BokenDinesh Kumar
Apr 5, 2018·Sensors·Lidia Nazaret Gómez-ArribasMaría Cruz Moreno-Bondi
Dec 22, 2019·Sensors·Monika NehraGiovanna Marrazza
Sep 22, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hongwei ZhangBoyang Yu
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Linglin FuYanbo Wang
Jul 22, 2021·Comprehensive Reviews in Food Science and Food Safety·Mati Ullah KhanZhenxing Li
Sep 16, 2021·Journal of Agricultural and Food Chemistry·Xianlong ZhangZhuoqun Su

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