Fluorescent in-situ hybridization on human embryos showing cleavage arrest after freezing and thawing

Human Reproduction
H LavergeM Dhont

Abstract

Our current freezing-thawing policy is to transfer only embryos that cleave further in the 24 h following thawing. The purpose of our study was to investigate the incidence of numerical abnormalities for chromosomes X, Y and 1 in blastomeres of human preimplantation embryos that survived cryopreservation but did not cleave further after thawing. A total of 63 embryos surviving a freezing-thawing cycle but not cleaving further within 24 h after thawing were screened. Of the 63 screened embryos that showed cleavage arrest 24 h after thawing, 56 embryos (88.9%) remained arrested 48 h after thawing and slightly more than half of these (29/56; 51.8%) showed further deterioration in morphological quality. Seven embryos (11.1%) showed signs of further cleavage; five embryos showed additional cleavage of one blastomere and two developed a blastocoelic cavity. Fluorescent in-situ hybridization (FISH) with three specific probes for simultaneous detection of chromosomes X, Y and 1 was performed and was successful in 60 out of 63 embryos. Of these successfully labelled embryos, 26 (43.3%) were in the diploid range: 12 (20%) were uniformly diploid for the chromosomes X, Y and 1; three embryos showed aneuploidy in all their blastomeres (two ...Continue Reading

Citations

Jul 12, 2002·Molecular Reproduction and Development·Kazuo GotoMichi Ebukuro
Jan 13, 2001·Molecular and Cellular Endocrinology·E Van den AbbeelA Van Steirteghem
May 1, 2007·Regenerative Medicine·Donald W LandryLauren Wiebe
Feb 5, 2008·Current Problems in Cardiology·Annarosa LeriWilliam H Frishman
Apr 22, 2004·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Andres SalumetsTimo Tuuri
Nov 3, 2004·The Journal of Clinical Investigation·Donald W Landry, Howard A Zucker
Aug 10, 1999·Luminescence : the Journal of Biological and Chemical Luminescence·P E Stanley, L J Kricka
Sep 27, 2005·Physiological Reviews·Annarosa LeriPiero Anversa
May 19, 2009·Reproductive Medicine and Biology·Eiko OtsuSeiji Ujiie

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