Fluorescent in situ hybridization shows DIPLOSPOROUS located on one of the NOR chromosomes in apomictic dandelions (Taraxacum) in the absence of a large hemizygous chromosomal region

Genome Génome / Conseil National De Recherches Canada
Radim J VašutHans de Jong

Abstract

Apomixis in dandelions (Taraxacum: Asteraceae) is encoded by two unlinked dominant loci and a third yet undefined genetic factor: diplosporous omission of meiosis (DIPLOSPOROUS, DIP), parthenogenetic embryo development (PARTHENOGENESIS, PAR), and autonomous endosperm formation, respectively. In this study, we determined the chromosomal position of the DIP locus in Taraxacum by using fluorescent in situ hybridization (FISH) with bacterial artificial chromosomes (BACs) that genetically map within 1.2-0.2 cM of DIP. The BACs showed dispersed fluorescent signals, except for S4-BAC 83 that displayed strong unique signals as well. Under stringent blocking of repeats by C0t-DNA fragments, only a few fluorescent foci restricted to defined chromosome regions remained, including one on the nucleolus organizer region (NOR) chromosomes that contains the 45S rDNAs. FISH with S4-BAC 83 alone and optimal blocking showed discrete foci in the middle of the long arm of one of the NOR chromosomes only in triploid and tetraploid diplosporous dandelions, while signals in sexual diploids were lacking. This agrees with the genetic model of a single dose, dominant DIP allele, absent in sexuals. The length of the DIP region is estimated to cover a regi...Continue Reading

References

Nov 11, 1995·Nucleic Acids Research·P VosM Kuiper
Jan 1, 1996·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·X B ZhongP Zabel
Sep 25, 1998·Science·N H Barton, B Charlesworth
Nov 18, 2000·Genome Génome / Conseil National De Recherches Canada·P van BaarlenJ H de Jong
Dec 29, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·B Charlesworth, D Charlesworth
Apr 23, 2002·Nature Reviews. Genetics·Sarah P Otto, Thomas Lenormand
May 30, 2002·Genome Génome / Conseil National De Recherches Canada·Paola LabombardaSergio Arcioni
Jul 2, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Peter J van Dijk
Sep 25, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M A BudimanR A Wing
Oct 18, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·K VijverbergP J Van Dijk
Dec 3, 2003·Molecular Genetics and Genomics : MGG·F PupilliS Arcioni
May 8, 2004·The Plant Cell·Ross A Bicknell, Anna M Koltunow
Feb 8, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ornella CalderiniFulvio Pupilli
Oct 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Andrew S CatanachRoss Bicknell
Aug 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Laksana KantamaHans de Jong
Dec 14, 2007·Annual Review of Genetics·Peggy Ozias-Akins, Peter J van Dijk
Feb 15, 2008·Nature·Maruthachalam RaviImran Siddiqi
Mar 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·David B Mark WelchMatthew Meselson
Feb 1, 1997·Genome Génome / Conseil National De Recherches Canada·M S ZwickT D McKnight
Aug 9, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Song-Bin ChangHans de Jong
Nov 15, 2008·Annual Review of Plant Biology·Damon Lisch
Feb 27, 2009·Current Opinion in Cell Biology·Marjori MatzkeAntonius J M Matzke
Mar 9, 2010·Nature·Vianey Olmedo-MonfilJean-Philippe Vielle-Calzada
Nov 9, 2010·Plant Signaling & Behavior·Noé Durán-Figueroa, Jean-Philippe Vielle-Calzada
Jan 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jesse D HollisterBrandon S Gaut
Feb 18, 2011·The Plant Cell·Manjit SinghDaniel Grimanelli
Mar 1, 2012·Current Biology : CB·Qi Zhou, Doris Bachtrog

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