Fluorescent in situ hybridization technique for cell type identification and characterization in the central nervous system

Methods : a Companion to Methods in Enzymology
Akiya WatakabeTetsuo Yamamori

Abstract

Central nervous system consists of a myriad of cell types. In particular, many subtypes of neuronal cells, which are interconnected with each other, form the basis of functional circuits. With the advent of genomic era, there have been systematic efforts to map gene expression profiles by in situ hybridization (ISH) and enhancer-trapping strategy. To make full use of such information, it is important to correlate "cell types" to gene expression. Toward this end, we have developed highly sensitive method of fluorescent dual-probe ISH, which is essential to distinguish two cell types expressing distinct marker genes. Importantly, we were able to combine ISH with retrograde tracing and antibody staining including BrdU staining that enables birthdating. These techniques should prove useful in identifying and characterizing the cell types of the neural tissues. In this article, we describe the methodology of these techniques, taking examples from our analyses of the mammalian cerebral cortex.

References

Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·F ClascáM Sur
Nov 15, 1996·Science·Y Tanabe, T M Jessell
Sep 1, 1997·Cerebral Cortex·Y Kawaguchi, Y Kubota
Jun 20, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A H HopmanE J Speel
Nov 11, 1998·Nature Medicine·P S ErikssonF H Gage
Feb 20, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E J SpeelP Komminoth
Jun 17, 2000·Progress in Neurobiology·C KöbbertS Thanos
Oct 7, 2003·The Journal of Comparative Neurology·Yasuyoshi ArimatsuAkira Omori
Aug 7, 2004·Science·Dave KosmanEthan Bier
Mar 18, 2006·Neuroscience Research·Zoltán Molnár, Amanda F P Cheung
Mar 21, 2006·Neuroscience Research·Tetsuo Yamamori, Kathleen S Rockland
May 23, 2006·Trends in Neurosciences·Sacha B NelsonChris M Hempel
Oct 27, 2006·Cerebral Cortex·Akiya WatakabeTetsuo Yamamori
Dec 8, 2006·Nature·Ed S LeinAllan R Jones
May 22, 2007·Nature Reviews. Neuroscience·Bradley J MolyneauxJeffrey D Macklis
Sep 8, 2007·Brain Research Reviews·M HelmstaedterB Sakmann
Apr 17, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Kouichi C NakamuraTakeshi Kaneko
Jun 24, 2008·Nature Reviews. Neuroscience·UNKNOWN Petilla Interneuron Nomenclature GroupRafael Yuste
Feb 20, 2009·Frontiers in Neuroscience·Andreas Burkhalter
Apr 22, 2009·Trends in Neurosciences·Pasko RakicMartin H Dominguez
Feb 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·M Victoria PuigYasuo Kawaguchi

❮ Previous
Next ❯

Citations

Jun 8, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Takashi KodamaSascha du Lac
Dec 17, 2015·Nature Communications·Alejandro LomnicziSergio R Ojeda
Oct 13, 2015·Nature Neuroscience·Min XuYang Dan
Jun 9, 2012·The Journal of Comparative Neurology·Akiya WatakabeTetsuo Yamamori
Apr 24, 2012·The Journal of Comparative Neurology·Brooke C Jarvie, Shane T Hentges
Jun 7, 2014·Frontiers in Systems Neuroscience·Akiya WatakabeTetsuo Yamamori
Jul 5, 2013·PloS One·Carrie E MahoneyEric L Bittman
Apr 22, 2016·The Journal of Comparative Neurology·Akiya Watakabe
Jun 23, 2012·Nature·Masaharu KinoshitaTadashi Isa
Oct 23, 2019·Proceedings of the National Academy of Sciences of the United States of America·Teppei EbinaMasanori Matsuzaki
Apr 18, 2020·The Journal of Comparative Neurology·Koshi MurataYugo Fukazawa

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.