PMID: 7538159Apr 26, 1995Paper

Fluorescent liposomes as quantitative markers of phagocytosis by alveolar macrophages

Journal of Immunological Methods
D G Perry, W J Martin

Abstract

A new phagocytic assay based on liposome ingestion by alveolar macrophages (AMs) is described. Fluorescent microspheres were encapsulated in liposomes, which allowed rapid enumeration by fluorometry. Liposomes made in the presence of vitronectin had the protein exposed on their outer surfaces, as determined by immunolabelling. Liposomes and rat AMs were incubated under conditions favorable for phagocytosis. Observation by light and electron microscopy showed AMs engulfing liposomes, with gradual transfer of fluorescent label from liposome to cell interior. This transfer was due to bona fide phagocytosis, as evidenced by (1) fluorescence of liposome-encapsulated dihydrofluorescein (DHF)-zymosan exclusively within AMs and (2) triggering of the respiratory burst by zymosan-associated liposomes only under conditions that allowed phagocytosis. Phagocytic activity was expressed as liposomes/cell, the average number of liposomes phagocytosed per macrophage. We used this technique to follow phagocytosis over time and to measure the effects of lipopolysaccharide (LPS) and vitronectin on AM phagocytosis.

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Citations

Dec 25, 2002·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Hubertus P A JersmannIan Dransfield
Mar 22, 2000·Journal of Immunological Methods·J O BrubakerP A Offit
Jan 23, 1998·Free Radical Biology & Medicine·C P TurnerO T Jones
Oct 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Prakash RamachandranJohn P Iredale
Nov 5, 1997·American Journal of Respiratory Cell and Molecular Biology·D G PerryW J Martin
Mar 6, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Keiji HirotaHiroshi Terada
Mar 17, 1999·Biochimica Et Biophysica Acta·F E GarrettR A Koch
Sep 1, 1999·The Journal of Pharmacy and Pharmacology·C R Dass, M A Burton

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