Fluorescent membrane probes' behavior in lipid bilayers: insights from molecular dynamics simulations

Biophysics Reviews
Luís M S Loura, J P Prates Ramalho

Abstract

Fluorescence spectroscopy and microscopy have been used as tools to study membrane biophysics for decades now. Because phospholipids are non-fluorescent, the use of extrinsic membrane probes in this context is commonplace. Two major points of concern arise regarding this matter, namely the incomplete understanding of the probe behavior inside the bilayer and the perturbation of the latter resulting from probe incorporation. To this effect, molecular dynamics (MD) simulations, by providing detailed atomic-scale information, represent a valuable way to characterize the location and dynamics of bilayer-inserted membrane probes, as well as the magnitude of perturbation they induce on the host lipid structure, and several important classes of reporter molecules have been studied in recent years. This article reviews the state of the art of MD simulations of bilayer-inserted fluorescent probes, focusing on the information that has been obtained from previous studies and hinting at future perspectives in this rapidly emerging field.

References

Jun 5, 1997·Nature·K Simons, E Ikonen
Apr 5, 2001·Biochimica Et Biophysica Acta·L M LouraM Prieto
Jul 3, 2002·Chemistry and Physics of Lipids·Olaf MaierDick Hoekstra
Jul 3, 2002·Chemistry and Physics of Lipids·Pentti Somerharju
Jan 7, 2004·Chemistry and Physics of Lipids·Soumi MukherjeeAmitabha Chattopadhyay
Nov 3, 2004·Biochimica Et Biophysica Acta·Walter L AshD Peter Tieleman
Feb 22, 2005·Biophysical Journal·Jarmila RepákováIlpo Vattulainen
Aug 4, 2006·The Journal of Physical Chemistry. B·Jarmila RepákováIlpo Vattulainen
Mar 29, 2007·The Journal of Physical Chemistry. B·Jana CurdováIlpo Vattulainen
Jun 13, 2008·Physical Chemistry Chemical Physics : PCCP·Ramachandra R GullapalliPeter J Butler
Aug 30, 2008·Chemistry and Physics of Lipids·Rodrigo F M de AlmeidaManuel Prieto
Sep 13, 2008·Biochimica Et Biophysica Acta·Yang ZhangErich Gulbins
Jan 21, 2009·Methods in Enzymology·Catherine A Royer, Suzanne F Scarlata

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Citations

Jun 29, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luís M S Loura, J P Prates Ramalho
Jul 7, 2010·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Biagio Lucini
Nov 19, 2019·Frontiers in Immunology·Nathaniel EnosEllen A G Chernoff
Dec 4, 2012·International Journal of Molecular Sciences·Luís M S Loura
Aug 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hugo A L FilipeLuís M S Loura
Feb 15, 2012·The Journal of Physical Chemistry. B·William K NitschkeHubert Stassen
Apr 4, 2013·The Journal of Physical Chemistry. B·David G AckermanGerald W Feigenson
Aug 27, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Robert GodinGonzalo Cosa
Oct 9, 2013·The Journal of Physical Chemistry. B·João R RobaloLuís M S Loura

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