Fluorescent Method for the Detection of Biothiols Using an Ag⁺-Mediated Conformational Switch

Sensors
Han ZhaoChangbei Ma

Abstract

In this work, a novel, simple, and time-saving fluorescence approach for the detection of biothiols (glutathione and cysteine) was developed by employing a DNA probe labeled with 2-aminopurine. As an adenine analogue, 2-aminopurine exhibits high fluorescence intensity that can be rapidly quenched in the presence of DNA. In the presence of Ag⁺, the fluorescence increased significantly, which was a result of the formation of cytosine⁻Ag⁺⁻cytosine base pairs and the release of 2-aminopurine. Upon addition of either glutathione or cysteine, the structure of cytosine⁻Ag⁺⁻cytosine was disrupted, a product of the stronger affinity between biothiols and Ag⁺. As a result, the 2-aminopurine-labeled DNA probe returned to its former structure, and the fluorescence signal was quenched accordingly. The detection limit for glutathione and cysteine was 3 nM and 5 nM, respectively. Furthermore, the determination of biothiols in human blood serum provided a potential application for the probe as a diagnostic tool in clinical practice.

References

Apr 11, 1992·Lancet·F J StaalL A Herzenberg
Jul 8, 2008·Molecular Aspects of Medicine·Shelly C Lu
Oct 3, 2008·Chemical Communications : Chem Comm·Akira OnoYoshiyuki Tanaka
Dec 31, 2009·Analytical Chemistry·Xue-Hui ZhouHan-Xi Shen
Mar 1, 2007·Indian Journal of Clinical Biochemistry : IJCB·S B PatilSheela M Kodliwadmath

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Citations

Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xi ZhouChangbei Ma

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Methods Mentioned

BETA
surface enhanced Raman scattering
fluorescence assay

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