Fluorescent microangiography (FMA): an improved tool to visualize the pulmonary microvasculature

Laboratory Investigation; a Journal of Technical Methods and Pathology
Andre E DutlyDavid W Courtman

Abstract

Visualization of the complex lung microvasculature and resolution of its three-dimensional architecture remains a difficult experimental challenge. We present a novel fluorescent microscopy technique to visualize both the normal and diseased pulmonary microvasculature. Physiologically relevant pulmonary perfusion conditions were applied using a low-viscosity perfusate infused under continuous airway ventilation. Intensely fluorescent polystyrene microspheres, confined to the vascular space, were imaged through confocal optical sectioning of 200 microm-thick lung sections. We applied this technique to rat lungs and the markedly enhanced depth of field in projected images allowed us to follow vascular branching patterns in both normal lungs and lungs from animals with experimentally induced pulmonary arterial hypertension. In addition, this method allowed complementary immunostaining and identification of cellular components surrounding the blood vessels. Fluorescent microangiography is a widely applicable and quantitative tool for the study of vascular changes in animal models of pulmonary disease.

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Citations

Sep 10, 2009·The Journal of Experimental Medicine·Lakshmi KugathasanDuncan J Stewart
Jan 11, 2012·The Journal of Clinical Investigation·Tania N Petruzziello-PellegriniPhilip A Marsden
Jul 10, 2013·Laboratory Investigation; a Journal of Technical Methods and Pathology·Takeshi MatsumotoSyota Sato
Aug 3, 2018·Stem Cells Translational Medicine·Timothy E G KruegerW Nathaniel Brennen
Oct 18, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Jasper H G HelthuisAlbert van der Zwan
May 13, 2010·European Journal of Nuclear Medicine and Molecular Imaging·Fabian KiesslingFrauke Alves
Jan 15, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jahar Bhattacharya
Jan 15, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nikolaos A ManiatisRichard D Minshall
Sep 17, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·James M HuntRubin M Tuder
Feb 9, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Walter FriesEmanuela Mazzon
Oct 17, 2015·Journal of Applied Physiology·Salil SharmaMansoureh Eghbali

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