Fluorescent Nanoparticles with Tissue-Dependent Affinity for Live Zebrafish Imaging

ACS Applied Materials & Interfaces
Deepak Kumar KhajuriaAharon Gedanken

Abstract

Carbon quantum dots (CDs) are widely investigated because of their low toxicity, outstanding water solubility, and high biocompatibility. Specifically, fluorescent CDs have attracted ever-increasing interest. However, so far, only a few studies have focused on assessing the fluorescence of nitrogen-doped CDs (N@CDs) during in vivo exposure. Here, we describe a strategy for low-cost, one-pot synthesis of N@CDs. The low toxicity and suitability of the N@CDs for fluorescence imaging are validated using zebrafish (ZF) as a model. Strong fluorescence emission from ZF embryos and larvae confirms the distribution of N@CDs in ZF. The retention of N@CDs is very stable, long lasting, and with no detectable toxicity. The presence of a strong fluorescence at the yolk sac, especially in the vicinity of the intestine, suggests that a high content of N@CDs entered the digestive system. This indicates that N@CDs may have potential imaging applications in elucidating different aspects of lipoprotein and nutritional biology, in a ZF yolk lipid transport and metabolism model. On the other hand, the presence of a strong selective fluorescence at the eyes and melanophore strips at the trunk and tail region of ZF larvae suggests that N@CDs has a hig...Continue Reading

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Citations

Feb 6, 2019·Nanomaterials·Cindy DiasBegoña Espiña
Jan 25, 2020·Advanced Healthcare Materials·Zhili PengRoger M Leblanc
May 10, 2020·Journal of Fish Biology·Deepak Kumar Khajuria, David Karasik
May 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nemi MalhotraChung-Der Hsiao
Jul 26, 2018·Nanomaterials·Enamul Haque, Alister C Ward
Jul 24, 2018·ACS Applied Materials & Interfaces·Fangman ChenYunkun Wu
May 22, 2018·ACS Applied Materials & Interfaces·Deepak Kumar KhajuriaDavid Karasik
Dec 14, 2018·Ultrasonics Sonochemistry·Vijay Bhooshan KumarOrit Shefi

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