Fluorescent probe partitioning in giant unilamellar vesicles of 'lipid raft' mixtures

The Biochemical Journal
Janos JuhaszFrances J Sharom

Abstract

Direct visualization of raft-like l(o) (liquid-ordered) domains in model systems and cells using microscopic techniques requires fluorescence probes with known partitioning preference for one of the phases present. However, fluorescent probes may display dissimilar partitioning preferences in different lipid systems and can also affect the phase behaviour of the host lipid bilayer. Therefore a detailed understanding of the behaviour of fluorescent probes in defined lipid bilayer systems with known phase behaviour is essential before they can be used for identifying domain phase states. Using giant unilamellar vesicles composed of the ternary lipid mixture DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine)/DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine)/cholesterol, for which the phase behaviour is known, we examined nine commonly used fluorescent probes using confocal fluorescence microscopy. The partitioning preference of each probe was assigned either on the basis of quantification of the domain area fractions or by using a well-characterized l(d) (liquid-disordered)-phase marker. Fluorescent probes were examined both individually and using dual or triple labelling approaches. Most of the probes partitioned individually into ...Continue Reading

References

Nov 27, 1987·Biochimica Et Biophysica Acta·J H IpsenM J Zuckermann
Jun 5, 1997·Nature·K Simons, E Ikonen
Jul 15, 1998·The Journal of Membrane Biology·D A Brown, E London
Feb 27, 2001·Biophysical Journal·C DietrichE Gratton
Feb 4, 2003·Journal of Lipid Research·Linda J Pike
May 9, 2003·The Journal of Biological Chemistry·Nicoletta KahyaPetra Schwille
Jul 9, 2004·Biophysical Journal·Philippe GirardPatricia Bassereau
Nov 27, 2004·Journal of Cell Science·Sebastian Schuck, Kai Simons
Apr 21, 2006·Nature Reviews. Molecular Cell Biology·John F Hancock
Oct 20, 2006·Nature Chemical Biology·Gerald W Feigenson
Oct 21, 2006·Nature Immunology·Andrey S Shaw
Oct 27, 2006·Biology of the Cell·Vera Michel, Marica Bakovic
Jan 19, 2007·The Journal of Physical Chemistry. B·Sarah L VeatchJenifer L Thewalt
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tobias BaumgartWatt W Webb
Jun 26, 2007·Biochimica Et Biophysica Acta·Tobias BaumgartGerald W Feigenson
Sep 13, 2007·The Journal of Biological Chemistry·Ana J García-SáezPetra Schwille
Oct 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sarah L VeatchKlaus Gawrisch
Feb 1, 2008·Biophysical Journal·Adam Cohen Simonsen
Jul 29, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Dolores C CarrerPetra Schwille
Jan 27, 2009·Biophysical Journal·James H DavisJanos Juhasz
May 6, 2009·Biophysical Journal·Alexander P DemchenkoAndrey S Klymchenko
Jul 28, 2009·Experimental Cell Research·Robert Lindner, Hassan Y Naim
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hermann-Josef KaiserKai Simons
Oct 9, 2009·The Biochemical Journal·Ilya LeventalPaul A Janmey
Jan 2, 2010·Science·Daniel Lingwood, Kai Simons

❮ Previous
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Citations

Apr 26, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Maria O OgunyankinMarjorie L Longo
Jun 15, 2011·Cold Spring Harbor Perspectives in Biology·Erdinc Sezgin, Petra Schwille
Dec 24, 2013·Chemistry and Physics of Lipids·Olga S OstroumovaLudmila V Schagina
Jun 19, 2013·Annual Review of Analytical Chemistry·Lothar OpilikRenato Zenobi
Jul 27, 2012·Molecular Membrane Biology·Erdinc Sezgin, Petra Schwille
Jan 25, 2013·Molecular Membrane Biology·Nadim ShadiacMaria Hrmova
Jul 30, 2014·Chemistry and Physics of Lipids·Evgeny G ChulkovOlga S Ostroumova
Mar 24, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Mihaela BacalumMarcel Ameloot
Oct 12, 2015·The Journal of Membrane Biology·Svetlana S EfimovaOlga S Ostroumova
Mar 28, 2012·Biochimica Et Biophysica Acta·Erdinc SezginPetra Schwille
Aug 28, 2010·The Biochemical Journal·Nicoletta Kahya
Dec 24, 2013·Chemistry & Biology·Andrey S Klymchenko, Rémy Kreder
Nov 20, 2012·Biochimica Et Biophysica Acta·Magali DeleuMarie-Paule Mingeot-Leclercq
Apr 23, 2011·Biochimica Et Biophysica Acta·Ilya Mikhalyov, Andrey Samsonov
Feb 14, 2012·Biophysical Journal·Juan M VanegasMarjorie L Longo
Apr 24, 2015·PloS One·Erdinc SezginPetra Schwille
Jun 9, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Yao-Wen JiangFu-Gen Wu
Jun 13, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Élodie BoisselierChristian Salesse
Jun 8, 2011·The Journal of Physical Chemistry. B·Michael J SkaugRoland Faller
Apr 3, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Joseph LorentMarie-Paule Mingeot-Leclercq
Feb 2, 2017·The Journal of Physical Chemistry. B·Sherry S W Leung, Jenifer Thewalt
Feb 24, 2017·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Elisabeth MantilTyler J Avis
Apr 4, 2017·Chemistry and Physics of Lipids·Ole G MouritsenAdam Cohen Simonsen
Apr 5, 2017·Advances in Colloid and Interface Science·Élodie BoisselierChristian Salesse
Feb 28, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Vinnie GaulTia E Keyes
Jun 13, 2015·Physical Chemistry Chemical Physics : PCCP·Hugo A L FilipeLuís M S Loura
Dec 1, 2017·PloS One·Svetlana S EfimovaOlga S Ostroumova
Jan 9, 2018·BMC Biophysics·Špela Zemljič JokhadarJure Derganc
Jan 13, 2018·Scientific Reports·Yan CuiPingshan Wang
Aug 27, 2018·Journal of Fluorescence·Hoa T HoangMichael U Kumke
Jan 9, 2020·Annual Review of Biophysics·Wade F ZenoJeanne C Stachowiak
Aug 26, 2020·Scientific Reports·Emilio J González-RamírezAlicia Alonso
Sep 19, 2020·Antioxidants·Eugene G MaksimovNikolai N Sluchanko
Jan 1, 2013·Faraday Discussions·Regina SchöpsJörg Kressler
Apr 16, 2020·Advanced Biosystems·Sebastian HimbertMaikel C Rheinstädter

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