Fluorescent probes for in vitro and in vivo quantification of hydrogen peroxide.

Chemical Science
Sen YeDan Yang

Abstract

Hydrogen peroxide (H2O2) plays essential roles in redox signaling and oxidative stress, and its dynamic concentration is critical to human health and diseases. Here we report the design, syntheses, and biological applications of HKPerox-Red and HKPerox-Ratio for quantitative measurement of H2O2. Both probes were successfully applied to detect endogenous H2O2 fluxes in living cells or zebrafish, and biological effects of multiple stress inducers including rotenone, arsenic trioxide, and starvation were investigated. As H2O2 is a common by-product for oxidase oxidation, a general assay was developed for ultrasensitive detection of various metabolites (glucose, uric acid, and sarcosine). Moreover, cellular H2O2 measurements were achieved for the first time by combining flow cytometry with live cell calibration. This study provides a pair of unique molecular tools for advanced H2O2 bio-imaging and assay development.

References

Feb 1, 1993·Free Radical Biology & Medicine·P C PanusB A Freeman
Nov 22, 2001·Journal of Bacteriology·L C Seaver, J A Imlay
Nov 26, 2002·Nature Immunology·Michael Reth
Feb 13, 2003·Science's STKE : Signal Transduction Knowledge Environment·Erica Werner
Sep 21, 2004·Annual Review of Plant Biology·Klaus Apel, Heribert Hirt
Nov 26, 2004·Journal of the American Chemical Society·Michelle C Y ChangChristopher J Chang
Mar 26, 2005·Brain Research. Molecular Brain Research·Claudia M TestaJ Timothy Greenamyre
May 26, 2005·Analytical Biochemistry·Krzysztof J ReszkaBradley E Britigan
Nov 25, 2005·Journal of the American Chemical Society·Evan W MillerChristopher J Chang
Mar 24, 2006·Nature Methods·Vsevolod V BelousovSergey Lukyanov
Apr 17, 2007·Molecular Cell·Elizabeth A VealBrian A Morgan
Jul 6, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nagendra Yadava, David G Nicholls
Aug 19, 2007·Nature Reviews. Molecular Cell Biology·Marco GiorgioPier Giuseppe Pelicci
Mar 14, 2008·Journal of the American Chemical Society·Duangkhae SrikunChristopher J Chang
Jul 9, 2008·Journal of the American Chemical Society·Bryan C Dickinson, Christopher J Chang
Dec 9, 2008·The Biochemical Journal·Michael P Murphy
May 13, 2010·Chemical Communications : Chem Comm·Zhaochao XuDavid R Spring
May 28, 2010·Autophagy·Michael DewaelePatrizia Agostinis
Jan 29, 2011·Environmental Health Perspectives·Caroline M TannerJ William Langston
Jun 23, 2011·Journal of the American Chemical Society·Masahiro AboTetsuo Nagano
Jul 20, 2011·Nature Chemical Biology·Bryan C Dickinson, Christopher J Chang
Jul 28, 2011·Chemical Communications : Chem Comm·Chul ChungBong Rae Cho
Aug 13, 2011·Accounts of Chemical Research·Alexander R LippertChristopher J Chang
Dec 14, 2011·Journal of the American Chemical Society·Lin YuanSasa Zhu
Dec 23, 2011·The Biochemical Journal·Jisun LeeJianhua Zhang
Jul 7, 2012·Free Radical Biology & Medicine·Baozhong ZhaoRonald P Mason
Jun 15, 2013·Chemical Communications : Chem Comm·Xiaolong SunTony D James
Sep 27, 2014·Cell Death and Differentiation·G FilomeniF Cecconi
Jun 4, 2015·Chemical Communications : Chem Comm·Amandeep KaurElizabeth J New
Apr 19, 2016·Nature Chemical Biology·Bruce MorganTobias P Dick
Jun 1, 2017·Chemistry : a European Journal·Diana AndinaPaola Luciani
Oct 22, 2017·Antioxidants & Redox Signaling·Flávia RezendeKatrin Schröder

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Methods Mentioned

BETA
flow cytometry
biosensors
confocal microscopy
Fluorescence
PMA
fluorescence imaging

Software Mentioned

HKPerox
FlowJo
Ratio
ImageJ

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