Fluorescent PSC-Derived Cardiomyocyte Reporter Lines: Generation Approaches and Their Applications in Cardiovascular Medicine.

Biology
Naeramit SontayananonKatja Gehmlich

Abstract

Recent advances have made pluripotent stem cell (PSC)-derived cardiomyocytes an attractive option to model both normal and diseased cardiac function at the single-cell level. However, in vitro differentiation yields heterogeneous populations of cardiomyocytes and other cell types, potentially confounding phenotypic analyses. Fluorescent PSC-derived cardiomyocyte reporter systems allow specific cell lineages to be labelled, facilitating cell isolation for downstream applications including drug testing, disease modelling and cardiac regeneration. In this review, the different genetic strategies used to generate such reporter lines are presented with an emphasis on their relative technical advantages and disadvantages. Next, we explore how the fluorescent reporter lines have provided insights into cardiac development and cardiomyocyte physiology. Finally, we discuss how exciting new approaches using PSC-derived cardiomyocyte reporter lines are contributing to progress in cardiac cell therapy with respect to both graft adaptation and clinical safety.

References

Jan 1, 1990·Annual Review of Cell Biology·C WilsonW J Gehring
May 23, 1998·Circulation Research·H KasaharaS Izumo
Oct 12, 2000·Biophysical Journal·S SankaranarayananT A Ryan
Jan 11, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M MüllerW M Franz
Feb 13, 2001·European Journal of Pharmacology·A Belus, E White
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·T NagaiA Miyawaki
Mar 17, 2001·Nature Reviews. Genetics·J A Robertson
Sep 21, 2002·Circulation Research·Chunhui XuMelissa K Carpenter
Mar 21, 2003·The Journal of Biological Chemistry·Susumu MinamisawaRumiko Matsuoka
Nov 5, 2003·Nature Biotechnology·Elizabeth A Jares-Erijman, Thomas M Jovin
Nov 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Georg NagelErnst Bamberg
Dec 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Milica RadisicGordana Vunjak-Novakovic
Jun 7, 2005·Expert Opinion on Biological Therapy·Andrea L Szymczak, Dario A A Vignali
Sep 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Valerie KouskoffGordon Keller
Aug 24, 2006·Nature Reviews. Molecular Cell Biology·Patrick Sung, Hannah Klein
Dec 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Atsuhiko T NaitoIssei Komuro
Apr 17, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Irit HuberLior Gepstein
Sep 27, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·David AndersonChris Denning
Nov 27, 2007·Nature Biotechnology·Stefan IrionGordon Keller
Dec 23, 2008·The Journal of Biological Chemistry·Jasper AkerboomEric R Schreiter
Dec 1, 2009·Nature Methods·Fumiyuki HattoriKeiichi Fukuda
Feb 26, 2010·Epigenetics & Chromatin·Fernando J Calero-NietoPeter N Cockerill
Jan 12, 2011·Cell Cycle·Anton A Komar, Maria Hatzoglou
Jan 19, 2011·Journal of Molecular Biology·Soeren TuranJuergen Bode
Jul 22, 2011·Stem Cells International·Ren MingliangWang Zhengguo
Oct 25, 2011·Nature Methods·David A ElliottEdouard G Stanley
Feb 22, 2012·Circulation Research·Todd J HerronJosé Jalife
Mar 13, 2012·Neuron·Christine Grienberger, Arthur Konnerth

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Methods Mentioned

BETA
Transgenic
transfection
fluorescence-activated cell sorting
FACS
biopsy

Software Mentioned

optogentics
Arclight
GECIs
GEVIs

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