Fluorescent Tobacco mosaic virus-Derived Bio-Nanoparticles for Intravital Two-Photon Imaging

Frontiers in Plant Science
Annette NiehlManfred Heinlein

Abstract

Multi-photon intravital imaging has become a powerful tool to investigate the healthy and diseased brain vasculature in living animals. Although agents for multi-photon fluorescence microscopy of the microvasculature are available, issues related to stability, bioavailability, toxicity, cost or chemical adaptability remain to be solved. In particular, there is a need for highly fluorescent dyes linked to particles that do not cross the blood brain barrier (BBB) in brain diseases like tumor or stroke to estimate the functional blood supply. Plant virus particles possess a number of distinct advantages over other particles, the most important being the multi-valency of chemically addressable sites on the particle surface. This multi-valency, together with biological compatibility and inert nature, makes plant viruses ideal carriers for in vivo imaging agents. Here, we show that the well-known Tobacco mosaic virus is a suitable nanocarrier for two-photon dyes and for intravital imaging of the mouse brain vasculature.

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Citations

Jun 24, 2016·Beilstein Journal of Nanotechnology·Claudia KochChristina Wege
Jan 16, 2020·International Journal of Molecular Sciences·Anubhab MukherjeeSudip Mukherjee
Apr 7, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Mehdi ShahgolzariSteven Fiering
May 7, 2019·Pharmaceutics·Esen SokulluMarc A Gauthier
Sep 9, 2017·Future Science OA·Kathleen Hefferon
Jul 6, 2019·Frontiers in Plant Science·Ahmad IbrahimRichard Kormelink
Oct 9, 2020·Current Medical Imaging·Alaa A A AljabaliMurtaza M Tambuwala

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Methods Mentioned

BETA
chemical modification
transmission
electrophoresis
fluorescence correlation spectroscopy
FCS
size exclusion centrifugation
electron microscopy

Software Mentioned

ZEN
GIS
IBiSA
Fiji

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