Fluorescently labeled microbubbles for facilitating translational molecular ultrasound studies

Drug Delivery and Translational Research
Patrick KoczeraTwan Lammers

Abstract

Microbubbles (MB) are routinely used as contrast agents for functional and molecular ultrasound (US) imaging. For molecular US imaging, MB are functionalized with antibodies or peptides, in order to visualize receptor expression by angiogenic or inflamed endothelium. In general, initial in vitro binding studies with targeted MB are performed using phase contrast microscopy. Difficulties in the identification of MB in standard phase contrast microscopy, however, generally result in high variability, high observer dependency, and low reproducibility. To overcome these shortcomings, we here describe a simple post-loading strategy for labeling polymer-based MB with fluorophores, and we show that the use of rhodamine-loaded MB in combination with fluorescence microscopy substantially reduces the variability and the observer dependency of in vitro binding studies. In addition, we demonstrate that rhodamine-loaded MB can also be used for in vivo and ex vivo experimental setups, e.g., for analyzing MB binding to inflamed carotids using two-photon laser scanning microscopy, and for validating the binding of VEGFR2-targeted MB to tumor endothelium. These findings demonstrate that fluorescently labeled MB substantially facilitate translat...Continue Reading

References

May 2, 2000·The Journal of the Acoustical Society of America·L HoffJ M Hovem
Apr 27, 2001·Radiology·R Weissleder, U Mahmood
Feb 4, 2003·Nature Reviews. Drug Discovery·Markus Rudin, Ralph Weissleder
Mar 12, 2003·Genes & Development·Tarik F Massoud, Sanjiv S Gambhir
Oct 25, 2003·Science·Harvey R Herschman
Mar 12, 2004·Journal of the American College of Cardiology·Andrew J HamiltonDavid D McPherson
Nov 21, 2007·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Alexander L Klibanov
Feb 10, 2009·Current Medicinal Chemistry·F KiesslingM Palmowski
Sep 3, 2009·Neoplasia : an International Journal for Oncology Research·Moritz PalmowskiFabian M Kiessling
Sep 29, 2009·Cardiovascular Research·Jonathan R Lindner
Oct 17, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Beat A KaufmannJonathan R Lindner
Sep 3, 2010·Investigative Radiology·Isabelle TardyMichel Schneider
May 7, 2011·Theranostics·Fabian KiesslingMoritz Palmowski
Sep 20, 2011·Ultrasound in Medicine & Biology·Stanley FokongJessica Gätjens

❮ Previous
Next ❯

Citations

Dec 10, 2013·Advanced Drug Delivery Reviews·Fabian KiesslingTwan Lammers
Mar 24, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Elizabeth HuynhGang Zheng
Jul 19, 2015·Biochemical and Biophysical Research Communications·Åsa BarrefeltMoustapha Hassan
Apr 4, 2015·Arteriosclerosis, Thrombosis, and Vascular Biology·Adelina CurajFabian Kiessling
Mar 9, 2021·Science China. Chemistry·Chen ZhangZhe Liu
Jun 27, 2020·Molecular Pharmaceutics·Mengjiao LiuTwan Lammers

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Related Papers

Journal of Controlled Release : Official Journal of the Controlled Release Society
Stanley FokongTwan Lammers
Ernst Schering Research Foundation Workshop
Alexander L Klibanov
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
Katsumasa Fujita
Nihon yakurigaku zasshi. Folia pharmacologica Japonica
Junichi Nabekura, Kei Eto
© 2021 Meta ULC. All rights reserved