PMID: 11929560Apr 4, 2002Paper

Fluoride and organic weak acids as respiration inhibitors for oral streptococci in acidified environments

Oral Microbiology and Immunology
T N PhanR E Marquis

Abstract

Oxygen metabolism (respiration) of Streptococcus mutans GS-5 involving NADH oxidases, mainly of the H(2)O-producing type, was found to be acid sensitive, as was NADH oxidase activity of cell extracts. Respiration of intact cells in acidified media was also highly sensitive to fluoride, with a 50% inhibitory concentration of about 0.02 mM at pH 4. In contrast, NADH oxidases in cell extracts were fluoride insensitive. Fluoride inhibition of respiration of intact cells was related to weak-acid effects leading to enhanced proton permeability of cells, cytoplasmic acidification and resultant acid inhibition of NADH oxidases and glycolysis. Organic weak acids, such as indomethacin and benzoate, were also effective inhibitors. H(2)O(2) production by intact cells of Streptococcus sanguis NCTC 10904, a peroxide producer, was similarly inhibited by fluoride or organic weak acids in acidified media. Thus, weak acids act as respiratory inhibitors for oral streptococci indirectly by acidifying the cytoplasm rather than acting as direct inhibitors of NADH oxidases.

References

Apr 1, 1991·Applied and Environmental Microbiology·W A Belli, R E Marquis
Jun 1, 1991·Oral Microbiology and Immunology·E V FrandsenM Kilian
Aug 1, 1986·Infection and Immunity·G R BenderR E Marquis
Dec 1, 1987·Journal of Dental Research·R M DuckworthA M Murray
Sep 1, 1985·Infection and Immunity·J CarlssonM B Edlund
Jul 15, 1983·Analytical Biochemistry·D A BenciniG A O'Donovan
Oct 1, 1982·Journal of Dental Research·C F Schachtele, M E Jensen
Feb 1, 1994·Oral Microbiology and Immunology·W A Belli, R E Marquis
Aug 1, 1995·Archives of Oral Biology·C S Ryan, I Kleinberg
Sep 1, 1995·Canadian Journal of Microbiology·W A BelliR E Marquis
Sep 1, 1995·Journal of Industrial Microbiology·R E Marquis
Apr 16, 1999·Oral Microbiology and Immunology·C E Caldwell, R E Marquis
Jan 12, 2000·Journal of Bacteriology·C M GibsonM G Caparon
Feb 3, 2000·Free Radical Biology & Medicine·L B PooleY Kamio
Feb 16, 2000·Oral Diseases·W H BowenT P Jerussi
Jun 13, 2000·Journal of Bacteriology·Y YamamotoY Kamio

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Citations

Feb 15, 2003·FEMS Microbiology Reviews·Robert E MarquisMarilaine Mota-Meira
Nov 14, 2006·Applied and Environmental Microbiology·Konstantinos PapadimitriouEffie Tsakalidou
Mar 18, 2010·Journal of Bacteriology·Jessica K KajfaszJosé A Lemos
Mar 2, 2011·Canadian Journal of Microbiology·Phuong T M Nguyen, Robert E Marquis
Aug 3, 2013·Clinical Oral Investigations·Nicole Birgit ArweilerThorsten Mathias Auschill

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