PMID: 40394Aug 1, 1979

Fluoride inhibition of protein and DNA synthesis in cells in vitro

Acta Pharmacologica Et Toxicologica
R I Holland

Abstract

Fluoride concentrations at and above 0.9 mM caused a progressive, concentration-related inhibition in the incorporation of both 14C-leucine and 3H-thymidine in LS cells incubated in medium with serum. The incorporation of leucine was more affected than that of thymidine. Lowering the pH enhanced the effect of fluoride on both. Removing serum from the incubation medium changed the effect of fluoride, particularly at low pH (7.0). Incorporation of leucine was then stimulated by low fluoride concentrations (0.5 and 0.9 mM), and the effect on thymidine incorporation was eradicated up to 1.3 mM-NaF. No differences were found in the pool and the specific activity of 14C-leucine in the fluoride exposed cells compared to control cells without fluoride (incubated at pH 7.4 in medium without serum). The cellular pool of 3H-thymidine decreased markedly during the incubation period, somewhat less in the fluoride exposed cells than in the control.

References

Jan 1, 1980·Acta Pharmacologica Et Toxicologica·C F HongsloR I Holland
Jun 4, 1999·The Journal of Nutrition·J L Borke, G M Whitford
Dec 22, 1999·Human & Experimental Toxicology·Magne RefsnesPer E Schwarze
Aug 23, 2006·Brazilian Dental Journal·Daniel Araki RibeiroDaisy Maria Fávero Salvadori
Nov 1, 1980·Acta Pharmacologica Et Toxicologica·J K HongsloR I Holland
Jan 1, 1980·Acta Pharmacologica Et Toxicologica·R I HollandT Christoffersen
Aug 29, 2014·Journal of Dietary Supplements·Sanjay S Karn, A V R L Narasimhacharya
Oct 1, 1985·Bulletin of Environmental Contamination and Toxicology·K S Pillai, U H Mane
Oct 1, 1979·Acta Pharmacologica Et Toxicologica·R I Holland
Apr 1, 1983·Acta Pharmacologica Et Toxicologica·C F HongsloR I Holland
Sep 17, 2008·Environmental Health Perspectives·Ramaswamy SharmaJohn D Bartlett
Jun 10, 2010·PloS One·Ramaswamy SharmaJohn D Bartlett
Jan 1, 1993·Environmental and Molecular Mutagenesis·E ZeigerK L Witt
Dec 29, 2004·Brazilian Oral Research·Daniel Araki RibeiroDaisy Maria Fávero Salvadori
Nov 1, 1983·Acta Pharmacologica Et Toxicologica·R I Holland
Jul 20, 2010·International Journal of Molecular Sciences·José A Morales-GonzálezManuel García-Luna Y González-Rubio

Citations

Jan 1, 1977·Archives of Oral Biology·J EkstrandS Rosell
May 1, 1979·Acta Pharmacologica Et Toxicologica·R I Holland, J K Hongslo
May 1, 1979·Acta Pharmacologica Et Toxicologica·R I Holland, J K Hongslo
Mar 1, 1978·Canadian Journal of Genetics and Cytology. Journal Canadien De Génétique Et De Cytologie·R MankovitzM Florian
Sep 1, 1978·Acta Pharmacologica Et Toxicologica·R I Holland, J K Hongslo
Sep 1, 1978·Acta Pharmacologica Et Toxicologica·J K Honglso, I Alfheim
Sep 1, 1976·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·B J LinE M Nagy
Aug 1, 1973·The Journal of Cell Biology·R Hand, I Tamm
Mar 1, 1974·Journal of Dental Research·J K HongsloJ Jonsen
Mar 25, 1974·Journal of Molecular Biology·J R Gautschi
Jan 1, 1969·Cold Spring Harbor Symposia on Quantitative Biology·C BaglioniM Jacobs-Lorena
Aug 28, 1964·Science·W R PROFFIT, J L ACKERMAN

Related Concepts

Fluoride Measurement
Thymidine
Protein Biosynthesis
Fluorides
Metabolic Inhibition
DNA Biosynthetic Process
Fibroblasts
DNA Replication
Fluoristat
Leucine

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.