PMID: 2119232Sep 1, 1990Paper

Fluoride-mediated activation of the respiratory burst in electropermeabilized neutrophils

Biochimica Et Biophysica Acta
P J Hartfield, J M Robinson

Abstract

Electropermeabilization creates small pores in the plasma membrane allowing the introduction of low-molecular-weight modulatory components, such as ions and nucleotides, into the cytosol. The present study investigates fluoride-mediated stimulation of the signal transduction pathway that activates the respiratory burst in electropermeabilized neutrophils. In marked contrast to intact (i.e., non-electropermeabilized) neutrophils, cells permeabilized by this technique demonstrated an immediate and potent stimulation of the superoxide (O2-)-generating NADPH oxidase in response to the addition of fluoride. Furthermore, permeabilization of neutrophils in the presence of exogenously added ATP enhanced the rate of F(-)-mediated O2- production. Fluoride-stimulated O2- production in electropermeabilized neutrophils was antagonized by GDP beta S and dependent upon the presence of Mg2+ in the medium, but was insensitive to pertussis toxin treatment, consistent with the hypothesis that fluoride activates a G protein, probably Gp, by interacting with the nucleotide-binding site on the G alpha subunit. In addition, electropermeabilized neutrophil O2- release triggered by F- was blocked by staurosporine and H-7, indicating that this pathway p...Continue Reading

References

Apr 1, 1979·The Journal of Clinical Investigation·J T CurnutteM L Karnovsky
Sep 21, 1989·Nature·M J Berridge, R F Irvine
Dec 1, 1989·Biochemical Genetics·N S SevilirW H Stone
Jul 26, 1988·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S Cockcroft, J Stutchfield
Nov 27, 1985·Biochemical and Biophysical Research Communications·C F Strnad, K Wong
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Mar 13, 1986·Biochemical and Biophysical Research Communications·T TamaokiF Tomita
Mar 15, 1986·The Biochemical Journal·D E Knight, M C Scrutton
Jan 1, 1986·Methods in Enzymology·J A Badwey, M L Karnovsky
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P C Sternweis, A G Gilman
Feb 1, 1954·The Journal of Experimental Medicine·R DULBECCO, M VOGT
Nov 17, 2012·Advances in Nutrition·Mario G FerruzziMarjorie R Freedman

❮ Previous
Next ❯

Citations

Mar 1, 1995·Histochemistry and Cell Biology·J M Robinson, J A Badwey
Jul 4, 2008·Histochemistry and Cell Biology·John M Robinson
Feb 19, 2009·Histochemistry and Cell Biology·John M Robinson
Oct 30, 1996·Journal of Immunological Methods·F R DeLeoM T Quinn
Jan 5, 2000·Journal of Immunological Methods·J M RobinsonT Takizawa
Jan 1, 1994·Progress in Histochemistry and Cytochemistry·K J HalbhuberN Zimmermann
Aug 1, 1997·Journal of Cellular Biochemistry·H C Birnboim, J K Sandhu
Dec 22, 1999·Human & Experimental Toxicology·M RefsnesP E Schwarze
Jul 1, 1997·Journal of Dental Research·L ShapiraM N Sela

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.