Abstract
Due to the advanced fluorescence property of N-carbon quantum dots (N-CQDs), a new method to detect pathogenic fungi by newly synthesized cornstalk N-CQDs modified with water-soluble amphotericin B (N-CQDs@AmpB) was developed. Specifically, N-CQDs with blue fluorescence were initially synthesized according to a previous report and modified with amphotericin B on their surfaces. Subsequently, the as-prepared N-CQDs@AmpB was used to detect Candida albicans, exhibiting a linear range of 2.60 × 105 to 1.99 × 108 cfu/mL and a detection limit of 1124 cfu/mL. Compared with other common methods, the method largely shortened the detection time and enabled the process to be performed with minimal interference from complex samples such as beef sausage. The high cost of water-soluble amphotericin B may hamper the large-scale application of the new detection method using N-CQDs@AmpB. Thus, alcohol-soluble amphotericin B was used in subsequent experiments, confirming its potential to broaden avenues for the detection of fungi.
References
Sep 1, 1995·Antimicrobial Agents and Chemotherapy·B CurrieM A Ghannoum
Sep 4, 1998·The European Respiratory Journal·A PachecoS Quirce
May 19, 2000·Antimicrobial Agents and Chemotherapy·R K LiM R McGinnis
Jul 25, 2007·Angewandte Chemie·Haipeng LiuChengde Mao
Mar 12, 2011·The Journal of Antimicrobial Chemotherapy·Vijay Kumar PrajapatiShyam Sundar
Aug 17, 2011·Nature Reviews. Microbiology·Peter E Sudbery
Mar 14, 2012·Chemical Communications : Chem Comm·Jianhua ShenChunzhong Li
Apr 4, 2014·The Analyst·Xiaoyue ShanHui Feng
Jan 6, 2015·European Biophysics Journal : EBJ·Barbara ChudzikMariusz Gagoś
Jul 15, 2015·Chemosphere·Rukshala AntonEnric Robine
Jul 21, 2015·Biosensors & Bioelectronics·Dan ZhongXiaoming Yang
Oct 22, 2015·Annual Review of Microbiology·Clarissa J Nobile, Alexander D Johnson
Jan 10, 2016·Journal of Dental Research·O SalvatoriM Edgerton
Mar 16, 2016·Toxicological Research·Anong BintvihokSungworn Usawang
Aug 4, 2016·Expert Review of Molecular Diagnostics·Matthew William McCarthy, Thomas J Walsh
May 4, 2017·Journal of Colloid and Interface Science·Mirza Muhammad Fahad Baig, Yu-Chie Chen
Mar 6, 2018·Bioconjugate Chemistry·Hang ZhangXianhong Wang
Jul 12, 2018·IEEE Transactions on Nanobioscience·Muhammad Waseem TahirWalter Lang
Citations
Aug 14, 2020·Nanomaterials·Rong GaoYunshan Liang
Oct 16, 2020·Critical Reviews in Analytical Chemistry·Mustafa Oguzhan CaglayanSamet Şahin
Jan 26, 2021·Food Chemistry·Yiheng ShiYahong Yuan
Apr 24, 2021·Nanomedicine·Mahboobeh JafariBahador Shahriarirad Sarkari