Fluorimetric determination of nicorandil in human plasma by a high-performance liquid chromatographic-postcolumn ultraviolet irradiation [corrected] system equipped with on-line back-pressure tubing

Journal of Chromatography. B, Biomedical Applications
Ken-ichi MawatariM Watanabe

Abstract

For the determination of nicorandil in plasma, a fluorometric technique using HPLC-postcolumn UV radiation [corrected] has been developed. The chromatographic system consisted of a single pump, photoreactor and on-line back-pressure tubing. The system was suitable for the separation of nicorandil under the present reaction conditions. The calibration graph was linear over the range 6.5-1170 ng ml-1 using an injected volume of 100 microliters. The pretreatment of the plasma samples consisted only of deproteinizing steps by adding perchloric acid. The mean recovery from plasma was 90.2%.

Citations

Jul 26, 2002·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Mitsuo Watanabe
Dec 2, 2010·Drug Testing and Analysis·Rajeev Jain, Rajeev Kumar Yadav

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