Fluorimetric high-performance liquid chromatography of prostaglandins and its application to their determination in human seminal fluid

Journal of Chromatography
M YamaguchiY Ohkura

Abstract

A highly sensitive and simple high-performance liquid chromatographic method with fluorescence detection for the determination of prostaglandins is described. Prostaglandins are converted into the corresponding fluorescent derivatives by reaction with 3-bromomethyl-6,7-dimethoxy-1-methyl-2(1H)-quinoxalinone in the presence of potassium hydrogen carbonate and 18-crown-6 in acetonitrile. The derivatives are separated simultaneously within 34 min (the total run time per injection, 56 min) on a reversed-phase column (YMC Pack C8) by a stepwise elution with mixtures of acetonitrile, methanol and water and detected fluorimetrically. The detection limits are 10-15 fmol at a signal-to-noise ratio of 5 in a 10-microliter injection volume. Prostaglandins E1, E2, F1 alpha and F2 alpha in human seminal fluids are measured by this method.

References

Mar 31, 1975·Science·H T JonssonD M Desiderio
Dec 13, 1985·Journal of Chromatography·C E MorrealD T Nemoto

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Citations

Sep 23, 1994·Journal of Chromatography. B, Biomedical Applications·Y OhkuraH Nohta
Mar 1, 1995·Free Radical Biology & Medicine·D Liu, L Li
Oct 24, 1998·Prostaglandins & Other Lipid Mediators·H JohnW Schlegel
Nov 21, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·H John, W Schlegel
Nov 1, 1989·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J SassardG Llorca
Oct 1, 1989·British Journal of Clinical Pharmacology·D BenzoniJ Sassard
Sep 1, 1987·Journal of Microscopy·K R Spring, P D Smith

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