Mar 18, 1976

Fluorimetric studies of tryptophyl exposure in concanavalin A

Biochimica Et Biophysica Acta
R Pelley, P Horowitz


Studies of the iodide ion quenching of the intrinsic fluorescence of Concanavalin A indicate that 50% of the tryptophyl fluorescence originates from exposed residues. This agrees with the X-ray crystallographic determination that two of the four tryptophan residues in a Concanavalin A monomer are on the surface. Previous studies have indicated that conformational changes induced by sugar binding alter the environment of aromatic residues. The present investigation finds that neither the specific binding of alpha-methyl-D-mannoside nor alteration of the Concanavalin A quaternary structure changes the number or accessibility of the solvent-exposed tryptophan residues. It therefore appears that the major conformational transitions in Concanavalin A do not affect steric access to the surface tryptophans and the effects previously observed may be ascribed to structurally internal tryptophan residues.

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Mentioned in this Paper

Hepatitis A
Fluorescence Spectroscopy
Plasma Protein Binding Capacity
X-Ray Diffraction
Protein Conformation
Hydrogen-Ion Concentration
Concanavalin A

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